Publication: Abnormal collagen deposition in synovia after collagen type V immunization in rabbits
Date
2008
Authors
Tsuzuki Ichicawa Ogido, Luciana ; Walcy Rosolia, Teodoro ; Pereira Velosa, Ana Paula ; De Oliveira, Cristiane Carla ; Roger Parra, Edwin ; Capelozzi, Vera Luiza ; Hajime Yoshinari, Natalino
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Sinovitis in Scleroderma (SSc) is rare,
usually aggressive and fully resembles rheumatoid
arthritis. Experimental models of SSc have been used in
an attempt to understand its pathogenesis. Previous
studies done in our laboratory had already revealed the
presence of a synovial remodeling process in rabbits
immunized with collagen V. To validate the importance
of collagen type V and to explore the quantitative
relationship between this factor and synovia remodeling
as well as the relationship between collagen type V and
other collagens, we studied the synovial tissue in
immunized rabbits. Rabbits (N=10) were immunized
with collagen V plus Freund’s adjuvant and compared
with animals inoculated with adjuvant only (N=10).
Synovial tissues were submitted to histological analysis,
immunolocalization to collagen I, III and V and
biochemical analysis by eletrophoresis, immunoblot and
densitometric method. The synovial tissue presented an
intense remodeling process with deposits of collagen
types I, III and V after 75 and 120 days of immunization,
mainly distributed around the vessels and interstitium of
synovial extracellular matrix. Densitometric analysis confirmed the increased synthesis of collagen I, III and
V chains (407.69±80.31; 24.46±2.58; 70.51±7.66,
respectively) in immunized rabbits when compared with
animals from control group (164.91±15.67; 12.89±1.05;
32±3.57) (p<0.0001). We conclude that synovial
remodeling observed in the experimental model can
reflect the articular compromise present in patients with
scleroderma. Certainly, this experimental model induced
by collagen V immunization will bring new insights in to pathogenic mechanisms and allow the testing of new
therapeutic strategies to ameliorate the prognosis for
scleroderma patients.
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