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Chromatin condensation but not DNA integrity of pig sperm is greater in the sperm-rich fraction

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dc.contributor.authorViñolas Vergés, Estel
dc.contributor.authorRibas Maynou, Jordi
dc.contributor.authorBarranco, Isabel
dc.contributor.authorPeres Rubio, Camila
dc.contributor.authorBonet, Sergi
dc.contributor.authorRoca, Jordi
dc.contributor.authorYeste, Marc
dc.contributor.departmentMedicina y Cirugía Animal
dc.date.accessioned2024-09-05T09:55:10Z
dc.date.available2024-09-05T09:55:10Z
dc.date.issued2023-11-06
dc.description©The Author(s) 2023. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in Journal of Animal Science and Biotechnology. To access the final edited and published work see https://doi.org/ https://doi.org/10.1186/s40104-023-00938-w
dc.description.abstractBackground protamination and condensation of sperm chromatin as well as DNA integrity play an essential role during fertilization and embryo development. In some mammals, like pigs, ejaculates are emitted in three separate fractions: pre-sperm, sperm-rich (SRF) and post sperm-rich (PSRF). These fractions are known to vary in volume, sperm concentration and quality, as well as in the origin and composition of seminal plasma (SP), with differences being also observed within the SRF one. Yet, whether disparities in the DNA integrity and chromatin condensation and protamination of their sperm exist has not been interrogated. Results This study determined chromatin protamination (Chromomycin A3 test, CMA3), condensation (Dibromobi‑mane test, DBB), and DNA integrity (Comet assay) in the pig sperm contained in the frst 10 mL of the SRF (SRF-P1), the remaining portion of the sperm-rich fraction (SRF-P2), and the post sperm-rich fraction (PSRF). While chromatin protamination was found to be similar between the diferent ejaculate fractions (P>0.05), chromatin condensation was seen to be greater in SRF-P1 and SRF-P2 than in the PSRF (P=0.018 and P=0.004, respectively). Regarding DNA integrity, no diferences between fractions were observed (P>0.05). As the SRF-P1 has the highest sperm concentra‑tion and ejaculate fractions are known to difer in antioxidant composition, the oxidative stress index (OSi) in SP, calcu‑ lated as total oxidant activity divided by total antioxidant capacity, was tested and confrmed to be higher in the SRFP1 than in SRF-P2 and PSRF (0.42±0.06 vs. 0.23±0.09 and 0.08±0.00, respectively; P<0.01); this index, in addition, was observed to be correlated to the sperm concentration of each fraction (Rs=0.973; P<0.001). Conclusion While sperm DNA integrity was not found to difer between ejaculate fractions, SRF-P1 and SRF-P2 were observed to exhibit greater chromatin condensation than the PSRF. This could be related to the OSi of each fraction.es
dc.formatapplication/pdfes
dc.format.extent11es
dc.identifier.citationJ Animal Sci Biotechnol 14, 139 (2023)
dc.identifier.doihttps://doi.org/10.1186/s40104-023-00938-w
dc.identifier.issnElectronic: 2049-1891
dc.identifier.urihttp://hdl.handle.net/10201/143622
dc.languageenges
dc.publisherBMC
dc.relationThis research was supported by the European Union’s Horizon 2020 research and innovation scheme under the Marie Skłodowska-Curie grant agreement No. 801342 (Tecniospring INDUSTRY; Grant: TECSPR-19-1-0003); the Ministry of Science and Innovation, Spain (Grants: PID2020-113320RB-I00, PID2020-113493RB-I00, RYC2021-034546-I and RYC2021-034764-I); the Catalan Agency for Management of University and Research Grants, Regional Government of Catalonia, Spain (Grants: 2017-SGR-1229 and 2021-SGR-00900); the Seneca Foundation, Regional Government of Murcia, Spain (Grant: 21935/PI/22); La Marató de TV3 Foundation (Grant: 214/857-202039); and the Catalan Institution for Research and Advanced Studies (ICREA).es
dc.relation.publisherversionhttps://jasbsci.biomedcentral.com/articles/10.1186/s40104-023-00938-wes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectChromatines
dc.subjectCondensation
dc.subjectDNA integrity
dc.subjectEjaculate fractions
dc.subjectPig
dc.subjectProtamination
dc.subjectSperm
dc.titleChromatin condensation but not DNA integrity of pig sperm is greater in the sperm-rich fractiones
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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