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Biological effects of silk fibroin 3D scaffolds on stem cells from human exfoliated deciduous teeth (SHEDs).

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dc.contributor.authorCollado-González, Mar
dc.contributor.authorPecci Lloret, María Pilar
dc.contributor.authorGarcía Bernal, David
dc.contributor.authorAznar-Cervantes, Salvador
dc.contributor.authorOñate Sánchez, Ricardo Elías
dc.contributor.authorMoraleda Jiménez, José María
dc.contributor.authorCenís, José L.
dc.contributor.authorRodríguez Lozano, Francisco Javier
dc.contributor.departmentDermatología, Estomatología, Radiología y Medicina Física
dc.date.accessioned2025-09-25T06:48:24Z
dc.date.available2025-09-25T06:48:24Z
dc.date.copyright© 2017
dc.date.issued2017-06-17
dc.description.abstractThe aim is to investigate in vitro biological effects of silk fibroin 3D scaffolds on stem cells from human exfoliated deciduous teeth (SHEDs) in terms of proliferation, morphological appearance, cell viability, and expression of mesenchymal stem cell markers. Silk fibroin 3D scaffolding materials may represent promising suitable scaffolds for their application in regenerative endodontic therapy approaches. SHEDs were cultured in silk fibroin 3D scaffolds. Then, cell numbers were counted and the Alamar blue colorimetric assay was used to analyse cell proliferation after 24, 48, 72, and 168 h of culture. The morphological features of SHEDs cultured on silk fibroin scaffolds were evaluated by scanning electron microscopy (SEM). Finally, cell viability and the expression of mesenchymal stem cell markers were analysed by flow cytometry. One-way analysis of variance (ANOVA) followed by a Bonferroni post-test was performed (P\0.05). At 24 and 48 h of culture, SHED proliferation on scaffolds was modest compared to the control although still significant (p\0.05). However, cell proliferation progressively increased from 72 to 168 h compared with the control (p\0.001; p\0.01). In addition, flow cytometry analysis showed that the culture of SHEDs on silk fibroin scaffolds did not significantly alter the level of expression of the mesenchymal markers CD73, CD90, or CD105 up to 168 h; in addition, cell viability in silk fibroin was similar to than obtained in plastic. Moreover, SEM studies revealed a suitable degree of proliferation, cell spreading, and attachment, especially after 168 h of culture. The findings from the current study suggest that silk fibroin 3D scaffolds had a favourable effect on the biological responses of SHEDs. Further in vivo investigations are required to confirm these results.
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dc.format.extent10
dc.identifier.citationOdontology, (2018) 106(2):125-134
dc.identifier.doihttps://doi.org/10.1007/s10266-017-0310-9
dc.identifier.eissn1618-1255
dc.identifier.issn1618-1247
dc.identifier.urihttp://hdl.handle.net/10201/159789
dc.languageeng
dc.publisherSpringer
dc.relationThis work was supported by the Spanish Net of Cell Therapy (TerCel) provided by Carlos III Institute of Health (ISCiii) (RETICS RD07/0010/2012 and RD12/0019/0001) together with the Junction Program for Biomedical Research in Advanced Therapies and Regenerative Medicine from ISCiii and FFIS
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s10266-017-0310-9
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleBiological effects of silk fibroin 3D scaffolds on stem cells from human exfoliated deciduous teeth (SHEDs).
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
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