Publication: Olig2 knockdown alleviates hypoxic- ischemic brain damage in newborn rats
Authors
Yang, Lijun ; Cui, Hong
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-344
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info:eu-repo/semantics/article
Description
Abstract
Objectives. Neuronal damage is an important
pathological mechanism in neonatal hypoxic-ischemic
brain damage (HIBD). We found in our previous studies
that oligodendrocyte transcription factor 2 (Olig2)
downregulation was able to increase cell survival in the
brain. However, the specific mechanism has yet to be
clarified.
Methods. Sprague-Dawley rats aged 3 d were
randomly divided into three groups: the normal control
group, the Olig2-RNAi group, and the RNAi-negative
control group. The normal control group received no
treatment, the Olig2-RNAi group received the Olig2
RNAi adenovirus, and the RNAi-negative control group
was given the control adenovirus after the completion of
the HIBD model. Infarct lesions and their volumes were
observed by triphenyltetrazolium chloride (TTC)
staining 3 d after the completion of the adenovirus local
injection. The condition of the tissue was characterized
by hematoxylin-eosin staining 7 d after the model was
established, and cell viability was determined by azure
methylene blue staining. Subcellular damage was
analyzed by transmission electron microscopy. Rotarod
analysis was performed to detect moving behavior
ability and an Morris water maze assay was conducted to
evaluate the memory.
Results. TTC staining showed a smaller brain injury
area in the Olig2-RNAi group than in the RNAi-negative
control group. Hematoxylin-eosin staining indicated the
presence of severe cell injury in the hippocampal region
after HIBD, which improved after Olig2 knockdown.
Azure methylene blue staining and electron microscopy
results suggested that the cells improved after Olig2
knockdown. The rats stayed longer on the rotating rod,
and their latency in the water maze test was gradually
shortened relative to that of the rats in the Olig2-RNAi
negative control group.
Conclusion. Olig2 knockdown can promote the
repair of hypoxic-ischemic brain damage in newborn
rats.
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