Publication: UV-Fixed-Thick-Blotch preparation improves sensitivity of Auramine staining
Date
2013
Authors
Muñoz Dávila, M.J. ; Martínez-Lirola, M.J. ; Cejudo-García, M.A.
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Publisher
American Society for Microbiology
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Description
© 2013, American Society for Microbiology.___ This document is the published version of a published work that appeared in final form in Journal of Clinical Microbiology___
To access the final edited and published work see:
https://doi.org/10.1128/jcm.00974-13
Abstract
We describe here a very simple modification of the auramine staining procedure based on preparation of a UV-fixed thick blotch
which allowed us to reach an overall sensitivity of 0.82 (592 acid-fast bacillus [AFB]-positive specimens/722 initial respiratory
specimens with positive mycobacterial culture) and sensitivities of 0.93 (526 AFB-positive specimens/564 culture-positive specimens)
for Mycobacterium tuberculosis complex and 0.42 (66 AFB-positive specimens/158 culture-positive specimens) for nontuberculous
mycobacteria.
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Citation
Journal of Clinical Microbiology, October 2013 Volume 51 Number 10 p. 3400–3402
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01-ene-2999
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