Publication: New in-depth analytical approach of the porcine seminal plasma proteome reveals potential fertility biomarkers
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Date
2018-02-07
Authors
Pérez Patiño, Cristina ; Parrilla Riera, Inmaculada ; Barranco Cascales, Isabel ; Vergara Barberán, María ; Simó Alfonso, Ernesto F. ; Herrero Martínez, José M. ; Rodríguez Martínez, Heriberto ; Martínez García, Emilio ; Roca Aleu, Jorge
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Publisher
American Chemical Society
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DOI
https://doi.org/10.1021/acs.jproteome.7b00728
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info:eu-repo/semantics/article
Description
Abstract
A complete characterization of the proteome of seminal plasma (SP) is an essential step to understand how SP influences sperm function and fertility after artificial insemination (AI). The purpose of this study was to identify which among characterized proteins in boar SP were differently expressed among AI boars with significantly different fertility outcomes. A total of 872 SP proteins, 390 of them belonging specifically to Sus Scrofa taxonomy, were identified (Experiment 1) by using a novel proteomic approach that combined size exclusion chromatography and solid-phase extraction as prefractionation steps prior to Nano LC-ESI-MS/MS analysis. The SP proteomes of 26 boars showing significant differences in farrowing rate (n = 13) and litter size (n = 13) after the AI of 10 526 sows were further analyzed (Experiment 2). A total of 679 SP proteins were then quantified by the SWATH approach, where the penalized linear regression LASSO revealed differentially expressed SP proteins for farrowing rate (FURIN, AKR1B1, UBA1, PIN1, SPAM1, BLMH, SMPDL3A, KRT17, KRT10, TTC23, and AGT) and litter size (PN-1, THBS1, DSC1, and CAT). This study extended our knowledge of the SP proteome and revealed some SP proteins as potential biomarkers of fertility in AI boars.
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Citation
Journal of Proteome Research, 2018, Vol. 17, N. 3, pp. 1065-1076
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1-ene-2999
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