Publication:
3D Immunofluorescence analysis of early thymic morphogenesis and medulla development

Simple item page
dc.contributor.authorMuñoz, Juan José
dc.contributor.authorCejalvo, Teresa
dc.contributor.authorTobajas, Esther
dc.contributor.authorFanlo, Lucía
dc.contributor.authorCortés, Alfonso
dc.contributor.authorZapata, Agustín Gregorio
dc.date.accessioned2020-04-21T12:00:27Z
dc.date.available2020-04-21T12:00:27Z
dc.date.issued2015
dc.description.abstractThe thymus represents an epithelial microenvironment specialized in the generation of Tcells. The mechanisms or signals that determine the initial differentiation of the two well distinguished histological compartments of the thymus, cortex and medulla, remain unknown. Here, we report a threedimensional analysis of the distribution of some established thymic epithelial markers in relation to thymic anatomical development during the first steps of thymus organogenesis. In the thymic primordium, initial lumen is lined by claudin (Cld)3/4+K5+ cells, after thymus growth and lobulation they form a continuous branched structure that increases its length and branching degree. Within it, the presence of luminal structures can be distinguished, even at E13.5. The medullary marker mouse thymic stroma 10 (MTS10) is upregulated in these Cld3/4+ lumen forming cells in a proximal-distal sequence. This structural organisation is histologically similar to that described in other epithelial organs undergoing a branching morphogenesis process. These results indicate that the thymic medulla can be evidenced as a continuous branched structure from early stages and suggest a thymic developmental program based on or containing elements of a branching morphogenesis program modified by the presence of lymphoid cells, in which medullary epithelial cell commitment is initially determined by lumen formation.es
dc.formatapplication/pdfes
dc.format.extent11es
dc.identifier.citationHistology and Histopathology, Vol. 30, n.º 5 (2015)
dc.identifier.doihttps://doi.org/10.14670/HH-30.589
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/90171
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectThymus organogenesises
dc.subjectThymic epitheliumes
dc.subjectMedullaes
dc.subjectBranching morphogenesises
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citologíaes
dc.title3D Immunofluorescence analysis of early thymic morphogenesis and medulla developmentes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Munoz-30-589-599-2015.pdf
Size:
27.05 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.39 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Histology and histopathology Vol.30, nº5 (2015)