Publication: 3D Immunofluorescence analysis of early
thymic morphogenesis and medulla development
Authors
Muñoz, Juan José ; Cejalvo, Teresa ; Tobajas, Esther ; Fanlo, Lucía ; Cortés, Alfonso ; Zapata, Agustín Gregorio
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
https://doi.org/10.14670/HH-30.589
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info:eu-repo/semantics/article
Description
Abstract
The thymus represents an epithelial
microenvironment specialized in the generation of Tcells. The mechanisms or signals that determine the
initial differentiation of the two well distinguished
histological compartments of the thymus, cortex and
medulla, remain unknown. Here, we report a threedimensional analysis of the distribution of some
established thymic epithelial markers in relation to
thymic anatomical development during the first steps of
thymus organogenesis. In the thymic primordium, initial
lumen is lined by claudin (Cld)3/4+K5+ cells, after
thymus growth and lobulation they form a continuous
branched structure that increases its length and
branching degree. Within it, the presence of luminal
structures can be distinguished, even at E13.5. The
medullary marker mouse thymic stroma 10 (MTS10) is
upregulated in these Cld3/4+ lumen forming cells in a
proximal-distal sequence. This structural organisation is
histologically similar to that described in other epithelial
organs undergoing a branching morphogenesis process.
These results indicate that the thymic medulla can be
evidenced as a continuous branched structure from early
stages and suggest a thymic developmental program
based on or containing elements of a branching
morphogenesis program modified by the presence of
lymphoid cells, in which medullary epithelial cell
commitment is initially determined by lumen formation.
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Citation
Histology and Histopathology, Vol. 30, n.º 5 (2015)
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