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Electron probe X-ray microanalysis of cisplatininduced cell death in rat pheochromocytoma PC12 cells

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dc.contributor.authorRamos, Juan M.
dc.contributor.authorArrebola, Francisco
dc.contributor.authorFernández Cervilla, Francisco J.
dc.contributor.authorCrespo, Vicente
dc.contributor.authorFernández Segura, Eduando
dc.date.accessioned2016-03-08T17:10:59Z
dc.date.available2016-03-08T17:10:59Z
dc.date.issued2011
dc.description.abstractSeveral lines of evidence suggest that cisplatin-induced cell death is not always the result of apoptosis. A distinctive feature between apoptosis and necrosis is the alteration in cell volume regulation and ion homeostasis. Here we analyzed the changes in intracellular element content during cell death induced by exposure to therapeutic concentrations of cisplatin in the PC12 cell line. To quantitate Na, Cl and K content, electron probe X-ray microanalysis (EPXMA) was performed in whole freeze-dried cells. We also traced the alterations in morphological features with fluorescence and transmission electron microscopy. EPXMA demonstrated progressive derangement of the absolute intracellular Na, Cl and K contents. Cisplatin-treated cells showed two microanalytical patterns: 1) cells with alterations in elemental content typical of apoptosis, i.e., an increase in intracellular Na and a decrease in intracellular Cl and K, and 2) cells characterized by an increase in Na content and a decrease in K content, with no changes in Cl content. This intracellular profile for Na, Cl, and K was not typical of necrosis or apoptosis. Morphological analysis revealed two cellular phenotypes: 1) cells characterized by a phenotype typical of apoptosis, and 2) cells characterized by a hybrid phenotype combining variable features of apoptosis and necrosis. Taken together, our findings suggest that therapeutic concentrations of cisplatin may cause a hybrid type of cell death characterized by concurrent apoptosis and necrosis in the same individual PC12 cell.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/48279
dc.languageenges
dc.publisherMurcia: F. Hernándezes
dc.relation.ispartofHistology and histopathology, Vol. 26, nº 3 (2011)es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectApoptosises
dc.subjectNecrosises
dc.subject.other61 - Medicinaes
dc.titleElectron probe X-ray microanalysis of cisplatininduced cell death in rat pheochromocytoma PC12 cellses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.26, nº3 (2011)