The Pseudoalteromonas luteoviolacea L-amino Acid Oxidase with Antimicrobial Activity Is a Flavoenzyme

Andreo-Vidal, Andrés; Sanchez-Amat, Antonio; Campillo-Brocal, Jonatan C.

Publication:
The Pseudoalteromonas luteoviolacea L-amino Acid Oxidase with Antimicrobial Activity Is a Flavoenzyme

dc.contributor.author	Andreo-Vidal, Andrés
dc.contributor.author	Sanchez-Amat, Antonio
dc.contributor.author	Campillo-Brocal, Jonatan C.
dc.contributor.department	Genética y Microbiología
dc.date.accessioned	2024-02-07T08:39:02Z
dc.date.available	2024-02-07T08:39:02Z
dc.date.issued	2018
dc.description	© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).	es
dc.description.abstract	The marine environment is a rich source of antimicrobial compounds with promising pharmaceutical and biotechnological applications. The Pseudoalteromonas genus harbors one of the highest proportions of bacterial species producing antimicrobial molecules. For decades, the presence of proteins with L-amino acid oxidase (LAAO) and antimicrobial activity in Pseudoalteromonas luteoviolacea has been known. Here, we present for the first time the identification, cloning, characterization and phylogenetic analysis of Pl-LAAO, the enzyme responsible for both LAAO and antimicrobial activity in P. luteoviolacea strain CPMOR-2. Pl-LAAO is a flavoprotein of a broad substrate range, in which the hydrogen peroxide generated in the LAAO reaction is responsible for the antimicrobial activity. So far, no protein with a sequence similarity to Pl-LAAO has been cloned or characterized, with this being the first report on a flavin adenine dinucleotide (FAD)-containing LAAO with antimicrobial activity from a marine microorganism. Our results revealed that 20.4% of the sequenced Pseudoalteromonas strains (specifically, 66.6% of P. luteoviolacea strains) contain Pl-laao similar genes, which constitutes a well-defined phylogenetic group. In summary, this work provides insights into the biological significance of antimicrobial LAAOs in the Pseudoalteromonas genus and shows an effective approach for the detection of novel LAAOs, whose study may be useful for biotechnological applications.	es
dc.format	application/pdf	es
dc.format.extent	16	es
dc.identifier.citation	Marine. Drugs 2018, 16(12), 499
dc.identifier.doi	https://doi.org/10.3390/md16120499
dc.identifier.issn	Electronic: 1660-3397
dc.identifier.uri	http://hdl.handle.net/10201/138822
dc.language	eng	es
dc.publisher	MDPI	es
dc.relation	Ministerio de Ciencia e Innovación, Spain, grant BIO2010-15226. Fundación Séneca, CARM, Spain, grant 11867/PI/09.	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	L-amino acid oxidase	es
dc.subject	Antimicrobial activity	es
dc.subject	Flavin cofactor	es
dc.subject	Pseudoalteromonas luteoviolacea	es
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiología	es
dc.title	The Pseudoalteromonas luteoviolacea L-amino Acid Oxidase with Antimicrobial Activity Is a Flavoenzyme	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es