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Matrix metalloproteinases and their role in the renal epithelial mesenchymal transition

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dc.contributor.authorAresu, L.
dc.contributor.authorBenali, S.
dc.contributor.authorGarbisa, S.
dc.contributor.authorGallo, E.
dc.contributor.authorCastagnaro, M.
dc.date.accessioned2016-03-08T16:42:44Z
dc.date.available2016-03-08T16:42:44Z
dc.date.issued2011
dc.description.abstractTubular cell epithelial-mesenchymal transition (EMT) is a fundamental contributor to renal fibrosis. The aim of this study was to investigate the activity of different matrix metalloproteinases by immunohistochemistry and gel-zymography in a model of chronic canine kidney disease. Immunohistochemistry for antibodies against MMP-9, MMP-2, MMP-13, MMP-14 and TIMP-2 was performed on 28 renal biopsy specimens. Selected cases were chosen for gelatin zymography. In moderate and severe tubulo-interstitial damage, increased expression of MMP-2 was noted. A peculiar staining pattern for MMP-2 in variable-sized vesicles, corresponding to the area of basement membrane splitting, was observed. The immunoexpression of MMP-9 and TIMP-2 was reduced in the same cases, compared to control dogs. The splitting of the membrane suggests an active role of this gelatinase in the disruption of type-IV collagen, the main basement membrane component, confirmed by MMP2 gelatinolytic activity by gel-zymography. These data could provide the basis for clinical trials examining the potential benefits of selective MMP-2 inhibitors in dogs with chronic kidney disease.es
dc.formatapplication/pdfes
dc.format.extent7es
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/48275
dc.languageenges
dc.publisherMurcia: F. Hernándezes
dc.relation.ispartofHistology and histopathology, Vol. 26, nº 3 (2011)es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectFibrosises
dc.subjectZymographyes
dc.subject.other636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticoses
dc.titleMatrix metalloproteinases and their role in the renal epithelial mesenchymal transitiones
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.26, nº3 (2011)