Publication: Matrix metalloproteinases and their role
in the renal epithelial mesenchymal transition
Authors
Aresu, L. ; Benali, S. ; Garbisa, S. ; Gallo, E. ; Castagnaro, M.
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Publisher
Murcia: F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Tubular cell epithelial-mesenchymal
transition (EMT) is a fundamental contributor to renal
fibrosis. The aim of this study was to investigate the
activity of different matrix metalloproteinases by
immunohistochemistry and gel-zymography in a model
of chronic canine kidney disease. Immunohistochemistry
for antibodies against MMP-9, MMP-2, MMP-13,
MMP-14 and TIMP-2 was performed on 28 renal biopsy
specimens. Selected cases were chosen for gelatin
zymography. In moderate and severe tubulo-interstitial
damage, increased expression of MMP-2 was noted. A
peculiar staining pattern for MMP-2 in variable-sized
vesicles, corresponding to the area of basement
membrane splitting, was observed. The immunoexpression
of MMP-9 and TIMP-2 was reduced in the
same cases, compared to control dogs. The splitting of
the membrane suggests an active role of this gelatinase
in the disruption of type-IV collagen, the main basement
membrane component, confirmed by MMP2
gelatinolytic activity by gel-zymography. These data
could provide the basis for clinical trials examining the
potential benefits of selective MMP-2 inhibitors in dogs
with chronic kidney disease.
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