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Assessment of the corneal collagen organization after chemical burn using second harmonic generation microscopy

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dc.contributor.authorBueno García, Juan Manuel
dc.contributor.authorÁvila, Francisco J.
dc.contributor.authorLorenzo-Martín, Elvira
dc.contributor.authorGallego-Muñoz, Patricia
dc.contributor.authorMartínez-García, M. Carmen
dc.contributor.departmentFísica
dc.date.accessioned2025-10-08T11:42:42Z
dc.date.available2025-10-08T11:42:42Z
dc.date.issued2021-01-11
dc.description© 2021, Optical Society of America under the terms of the OSA Open Access Publishing Agreement
dc.description.abstractThe organization of the corneal stoma is modified due to different factors, including pathology, surgery or external damage. Here the changes in the organization of the corneal collagen fibers during natural healing after chemical burn are investigated using second harmonic generation (SHG) imaging. Moreover, the structure tensor (ST) was used as an objective tool for morphological analyses at different time points after burn (up to 6 months). Unlike control corneas that showed a regular distribution, the collagen pattern at 1 month of burn presented a non-organized arrangement. SHG signal levels noticeably decreased and individual fibers were hardly visible. Over time, the healing process led to a progressive re-organization of the fibers that could be quantified through the ST. At 6 months, the stroma distribution reached values similar to those of control eyes and a dominant direction of the fibers re-appeared. The present results show that SHG microscopy imaging combined with the ST method is able to objectively monitor the temporal regeneration of the corneal organization after chemical burn. Future implementations of this approach into clinically adapted devices would help to diagnose and quantify corneal changes, not only due to chemical damages, but also as a result of disease or surgical procedures.
dc.formatapplication/pdf
dc.format.extent10
dc.identifier.citationBiomed. Opt. Express 12, 756-765 (2021)
dc.identifier.doihttps://doi.org/10.1364/BOE.412819
dc.identifier.eissn2156-7085
dc.identifier.urihttp://hdl.handle.net/10201/165189
dc.languageeng
dc.publisherOptica Publishing Group
dc.relationInstituto de Salud Carlos III (PI15/01906); Fundación Séneca (19897/GERM/15).
dc.relation.publisherversionhttps://opg.optica.org/boe/fulltext.cfm?uri=boe-12-2-756
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleAssessment of the corneal collagen organization after chemical burn using second harmonic generation microscopy
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
relation.isAuthorOfPublication460f1110-b358-474a-9e08-f92fabe4be2b
relation.isAuthorOfPublication.latestForDiscovery460f1110-b358-474a-9e08-f92fabe4be2b
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