Publication:
Measurement of cortisol, cortisone and 11β-hydroxysteroid dehydrogenase type 2 activity in hair of sows during different phases of the reproductive cycle

Simple item page
dc.contributor.authorLópez Arjona, Marina
dc.contributor.authorTecles, Fernando
dc.contributor.authorMateo, Sandra V
dc.contributor.authorContreras Aguilar, María Dolores
dc.contributor.authorMartínez Miró, Silvia
dc.contributor.authorCerón, José J.
dc.contributor.authorMartínez Subiela, Silvia
dc.contributor.departmentMedicina y Cirugía Animal
dc.date.accessioned2024-02-07T12:20:58Z
dc.date.available2024-02-07T12:20:58Z
dc.date.issued2020-04-29
dc.description©2020. This document is the published, version of a Published Work that appeared in final form in The Veterinary Journal. To access the final edited and published work see https://doi.org/10.1016/j.tvjl.2020.105458
dc.description.abstractTwo sensitive assays based on AlphaLISA technology were developed and validated for the measurement of cortisol and cortisone in hair of pigs, that also enabled estimation of 11 beta-hydroxysteroid dehydrogenase type 2 activity. These assays were applied to hair samples from sows (n = 32) collected at 5 days before, and at 23 and 59 after farrowing, in reproductive cycles in two different periods: spring-summer (n = 16) and winter-spring (n = 16). The assays were precise (imprecision <12%) and accurate (recovery range, 80-115%) for cortisol and cortisone determination. Hair cortisone concentrations and the cortisone/cortisol ratio (an estimate of 11 beta-hydroxysteroid dehydrogenase isoenzyme type 2 activity) increased after farrowing more than cortisol, being these changes of higher magnitude during periods of higher atmospheric temperature. The measurement of hair cortisone concentrations and estimations of the activity of the 11 beta-hydroxysteroid dehydrogenase isoenzyme type 2, measured by the assays developed in this study, are complementary biomarkers to hair cortisol, and can increase at periods associated with stress, such as farrowing and lactation, especially at high atmospheric temperatureses
dc.embargo.termsSi
dc.formatapplication/pdfes
dc.format.extent9
dc.identifier.doihttps://doi.org/10.1016/j.tvjl.2020.105458
dc.identifier.eisbnThe Veterinary Journal, 259-260 (2020): 105458es
dc.identifier.issnPrint: 1090-0233
dc.identifier.urihttp://hdl.handle.net/10201/138883
dc.languageenges
dc.publisherElsevieres
dc.relationMinisterio de Economía, Industria y Competitividad’ (AGL2016-79096-R)es
dc.rightsinfo:eu-repo/semantics/embargoedAccesses
dc.subject11 beta-hydroxysteroid dehydrogenasees
dc.subjectCortisoles
dc.subjectCortisonees
dc.subjectHaires
dc.subjectStresses
dc.titleMeasurement of cortisol, cortisone and 11β-hydroxysteroid dehydrogenase type 2 activity in hair of sows during different phases of the reproductive cyclees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
1-s2.0-S1090023320300356-main.pdf
Size:
1.67 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.26 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos