Publication: Characterization of natural occurring Pneumocystis
carinii pneumonia in pigs by histopathology, electron
microscopy, in situ hybridization and PCR amplification
Loading...
Date
1998
Authors
Ramos Vara, J. A. ; Lu, J. J. ; da Silva, A. J. ; Montone, K. T. ; Pieniazek, N. J. ; Lee, C. H. ; Pérez, L. ; Steficek, B. A. ; Dunstan, R. W. ; Craft, D. ; Watson, G. L.
item.page.secondaryauthor
item.page.director
Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Macroscopic. histolog ic . ultrastruc tural.
microbiologic. ill sirll hybridi za ti on (ISH ) and PCR
detection results in three 8-week-old pi gs naturall y
infected with Pllel.llllonsris c{lrinii (PC) are described .
All an imals had a nonsu ppurati ve interstitial pneumoni a
and intra-alveolar PlleulIlucysris organisms with foamy
eos in ophili c and PAS positi ve appearance . Ultrastructurally. PC trophozoites and cysts we re observed in
pigs No.2 and NO .3. with the fo rmer being much more
numerous. PC organisms we re located on the alveolar
surface or within the alveolar septa . Trophozoites had
numerous filopodia and were thin-wall ed. Cysts had no
o r few filopodia. we re thick- wa ll ed and co nt a ined
intracysti c bodies. Using non-isotopic ISH on formalinfixed. paraffin-embedded lung tissue sections. PC DNA
from pigs No.2 and No . 3 hyb ridi zed with a probe
specific for PC ribosomal RNA (rRNA). Using primers
spec ific for mit oc ho ndrial rRNA ge ne (pA Z I0 2-
E/pAZ I 02-H). and for the interna l transcriber space rs of
ribosomal gene of PC. PCR methods amplified a product
in the lung of pigs No.2 and No.3 using either frozen or
formalin -fi xed and paraffin -embedded lung tissue. DNA
from Pig No. I samples did not amplify with any primer.
This is the first time that molecular biology techniques
(ill siru hybridi zation and PCR) have been applied to the
study of porc ine pneumocystosis.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. CC BY 4.0