Publication: Antioxidant enzymes in renal cell carcinoma
Authors
Soini, Y. ; Kallio, J.P. ; Hirvikoski, P. ; Helin, H. ; Kellokumpu-Lehtinen, P. ; Tammela, T.L.J. ; Peltoniemi, M. ; Martikainen, P.M. ; Kinnula, V.L.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The aim of the study was to estimate the
significance of oxidative/nitrosative damage and
expression of antioxidant enzymes in renal cell
carcinomas (RCC). For this we investigated
immunohistochemically six antioxidant enzymes
(AOEs) including MnSOD, ECSOD, thioredoxin,
thioredoxin reductase, and gammaglutamyl cysteine
synthetase heavy and light chain in 138 RCCs. As an
indicator of oxidative/nitrosative damage, sections were
stained with an antibody to nitrotyrosine. The extent of
apoptosis was evaluated by TUNEL method and
proliferation by immunohistochemistry to Ki67. Variable
expression of all AOEs could be seen in RCC with
expression of MnSOD being strongest. Nitrotyrosine
was significantly associated with high grade tumors.
MnSOD was associated with tumors of a lower stage.
Cases showing ECSOD reactivity had higher and cases
expressing thioredoxin lower apoptotic index than other
tumors. No association with patient prognosis was
observed. According to the results renal cell carcinomas
show oxidative/nitrosative damage which, according to
nitrotyrosine staining, was higher in high grade tumors.
Of AOEs, MnSOD was more abundantly expressed in
low stage tumors suggesting that its antioxidant function
could play a main role to prevent development of
oxidative damage leading to more aggressive tumors.
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