Publication:
Use of photodynamic therapy and chitosan for inactivacion of Candida albicans in a murine model

Thumbnail Image
Files
Use of photodynamic therapy and chitosan for inactivation of Candida albicans in a murine model.pdf(665.49 KB)
Date
2016-09-03
relationships.isAuthorOfPublication
relationships.isSecondaryAuthorOf
relationships.isDirectorOf
Authors
Camacho Alonso, Fabio ; Martínez Beneyto, Yolanda ; Gallego, Mª Carmen ; Cuello, Francisco ; Buendía, Antonio Julián ; Pérez Lajarín, Leonor ; Salinas, Jesús
item.page.secondaryauthor
item.page.director
Publisher
Wiley
publication.page.editor
publication.page.department
Dermatología, Estomatología, Radiología y Medicina Física
DOI
https://doi.org/10.1111/jop.12435
item.page.type
info:eu-repo/semantics/article
Description
© 2016 John Wiley & Sons A/S. This document is the Submitted, Accepted, Published, version of a Published Work that appeared in final form in Journal of Oral Pathology & Medicine. To access the final edited and published work see https://doi.org/10.1111/jop.12435
Abstract
Background The wide use of topical and systemic antifungal agents as the conventional treatment for oral candidiasis has caused Candida albicans to develop resistance to these agents. The aims of this study were to evaluate the effects of photodynamic therapy (PDT) and chitosan on buccal candidiasis and study the possible enhancive effect of chitosan on the photosensitizer methylene blue. Methods Thirty-five DBA/2 immunosuppressed mice were orally inoculated with a suspension of Candida albicans. The animals were randomized into seven groups (n = 5 per group): Group 1 (control); Group 2 (nystatin); Group 3 (PDT); Group 4 (chitosan 1.5 mg/ml); Group 5 (chitosan 3 mg/ml); Group 6 (PDT + chitosan 1.5 mg/ml); and Group 7 (PDT + chitosan 3 mg/ml). The Candida albicans count was evaluated on days 3, 5, 7, and 11 after inoculation. At last, macroscopic and microscopic analyses of the tongue dorsa were performed. Results On day 7 after inoculation, the control group showed a greater number of Candida albicans (5.25 ± 0.41 log10 CFU/ml), with significant differences compared to all other groups (P ≤ 0.05). On day 11 after inoculation, animals treated with PDT showed lower CFU/ml count. Groups 2, 3, 4, 5, and 6 showed fewer microscopic candidiasis lesions than Groups 1 and 7. Conclusions PDT has an antifungal effect, even greater than nystatin. Chitosan has a powerful fungicide effect but did not possess any enhancive effect on methylene blue.
publication.page.subject
Candida albicans , Chitosan , Murine model , Oral candidiasis , Photdynamic therapy
Citation
Journal of Oral Pathology & Medicine, 2016, Vol. 45, Issue 8, pp. 627-633
URI
http://hdl.handle.net/10201/146362
item.page.embargo
SI
Collections
Artículos
Ir a Estadísticas