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In vitro bacteriological effect of tri-beveled needle electrolysis against Staphylococcus aureus

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dc.contributor.authorGarcía Vidal, José Antonio
dc.contributor.authorSalinas, Jesús
dc.contributor.authorOrtega, Nieves
dc.contributor.authorEscolar Reina, Pilar
dc.contributor.authorCamacho Alonso, Fabio
dc.contributor.authorMedina Mirapeix, Francesc
dc.contributor.departmentFisioterapia
dc.date.accessioned2025-01-20T13:03:51Z
dc.date.available2025-01-20T13:03:51Z
dc.date.issued2022-07-06
dc.description© 2022 The authors. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This document is the Published version of a Published Work that appeared in final form in Scientific Reports. To access the final edited and published work see https://doi.org/10.1038/s41598-022-15666-wes
dc.description.abstractPercutaneous needle electrolysis using tri-beveled needles with a specific protocol (5 mA applied for 25 s) has demonstrated to provoke a clinical reduction of recurrent bacterial infections in mammary fistulas. However, the bactericidal effect of needle electrolysis in this pathology remains theoretical. This in vitro study evaluated the bactericidal effect of this protocol and whether it changed when introducing small variations. Staphylococcus aureus were generated in saline solution (9 Log10 CFU/mL) and treated in three different experiments including the main protocol and introducing variations in needle gauge, intensity, and total dosage, respectively. After 24 h, the viable cell count showed that the protocol had an average reduction of 5 log10 CFU/ml compared to the control group. While variations in needle gauge did not modify this effect, variations in current intensity or dosage did. This study demonstrated that the bacterial effect was greater by increasing either current intensity or total dosage, and it decreased with substantial reductions of these parameters.es
dc.formatapplication/pdfes
dc.format.extent9es
dc.identifier.doihttps://doi.org/10.1038/s41598-022-15666-w
dc.identifier.eisbnScientific Reports, 2022, Vol. 12 : 11468es
dc.identifier.issnElectronic: 2045-2322
dc.identifier.urihttp://hdl.handle.net/10201/148843
dc.languageenges
dc.publisherNature Researches
dc.relationSin financiación externa a la Universidades
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-022-15666-wes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectInfectiones
dc.subjectInflammationes
dc.subjectMicrobiology techniqueses
dc.subjectTranslational researches
dc.titleIn vitro bacteriological effect of tri-beveled needle electrolysis against Staphylococcus aureuses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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