Publication:
A quantitative description of the insulin-induced ultrastructural changes in newborn rat hepatocytes

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Authors
Maintas, D.B. ; Kotoulas, Othon B. ; Kotoulas, Angeliki O.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The effects of insulin on the ultrastructure of newborn rat hepatocytes were systernatically quantified at satisfactory statistical significance. Insulin prevented the normal postnatal increase in the total volume of lysosomes and the breakdown of glycogen inside these organelles. The lysosomal glycogen-hydrolysing enzyme, acid alpha 1,4 glucosidase was inhibited by the hormone. Insulin also prevented the normal postnatal increase in the total volume of peroxisomes, especially of the crystalloid core-devoid type. The hormone produced an increase in the area of cell membrane, due to the forrnation of many irregular folds of the cell surface. These results constitute good evidence for participation of lysosomes and peroxisomes in the overall glycogen degradation and or gluconeogenesis in the newborn rat hepatocytes.
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