Effect of terbinafine on the biosynthetic pathway of isoprenoid compounds in carrot suspension cultured cells

Almagro Romero, Lorena; Miras Moreno, Begoña; Pedreño García, María Ángeles; Sabater Jara, Ana Belén

Publication:
Effect of terbinafine on the biosynthetic pathway of isoprenoid compounds in carrot suspension cultured cells

dc.contributor.author	Almagro Romero, Lorena
dc.contributor.author	Miras Moreno, Begoña
dc.contributor.author	Pedreño García, María Ángeles
dc.contributor.author	Sabater Jara, Ana Belén
dc.contributor.department	Biología Vegetal
dc.date.accessioned	2025-05-15T09:51:24Z
dc.date.available	2025-05-15T09:51:24Z
dc.date.issued	2018-04-21
dc.description	© 2018, Springer-Verlag GmbH Germany. This document is the Published Manuscript version of a Published Work that appeared in final form in Plant Cell Reports. To access the final edited and published work see https://doi.org/10.1007/s00299-018-2287-4
dc.description.abstract	Plant sterols are essential components of membrane lipids, which contributing to their fluidity and permeability. Besides their cholesterol-lowering properties, they also have anti-inflammatory, antidiabetic and anticancer activities. Squalene, which is phytosterol precursor, is widely used in medicine, foods and cosmetics due to its anti-tumor, antioxidant and anti-aging activities. Nowadays, vegetable oils constitute the main sources of phytosterols and squalene, but their isolation and purification involve complex extraction protocols and high costs. In this work, Daucus carota cell cultures were used to evaluate the effect of cyclodextrins and terbinafine on the production and accumulation of squalene and phytosterols as well as the expression levels of squalene synthase and cycloartenol synthase genes. D. carota cell cultures were able to produce high levels of extracellular being phytosterols in the presence of cyclodextrins (12 mg/L), these compounds able to increase both the secretion and accumulation of phytosterols in the culture medium. Moreover, terbinafine induced a significant increase in intracellular squalene production, as seen after 168 h of treatment (497.0 ± 23.5 µg g dry weight−1) while its extracellular production only increased in the presence of cyclodextrins.The analysis of sqs and cas gene expression revealed that cyclodextrins did not induce genes encoding enzymes involved in the phytosterol biosynthetic pathway since the expression levels of sqs and cas genes in cyclodextrin-treated cells were lower than in control cells. The results, therefore, suggest that cyclodextrins were only able to release phytosterols from the cells to the extracellular medium, thus contributing to their acumulation. To sum up, D. carota cell cultures treated with cyclodextrins or terbinafine were able to produce high levels of phytosterols and squalene, respectively, and, therefore, these suspension-cultured cells of carrot constitute an alternative biotechnological system, which is at the same time more sustainable, economic and ecological for the production of these bioactive compounds.
dc.format	application/pdf	es
dc.format.extent	9
dc.identifier.citation	Plant Cell Reports, 2018, Vol. 37, pp. 1011–1019
dc.identifier.doi	https://doi.org/10.1007/s00299-018-2287-4
dc.identifier.issn	Print: 0721-7714
dc.identifier.issn	Electronic: 1432-203X
dc.identifier.uri	http://hdl.handle.net/10201/154670
dc.language	eng	es
dc.publisher	Springer
dc.relation	Ministerio de Economía y Competitividad (no. BIO2014-51861-R), Fundación Seneca-Agencia de Ciencia y Tecnología de la Región de Murcia (no. 19876/GERM/15).	es
dc.relation.publisherversion	https://link.springer.com/article/10.1007/s00299-018-2287-4
dc.rights	info:eu-repo/semantics/embargoedAccess	es
dc.subject	Cyclodextrins
dc.subject	Carrot suspension cultured cells
dc.subject	Gene expression
dc.subject	Phytosterols
dc.subject	Squalene
dc.title	Effect of terbinafine on the biosynthetic pathway of isoprenoid compounds in carrot suspension cultured cells	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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