Functionalized Nanoplastics (NPs) Increase the Toxicity of Metals in Fish Cell Lines

González Fernández, Carmen; Díaz Baños, F. Guillermo; Esteban Abad, María Ángeles; Cuesta, Alberto

Publication:
Functionalized Nanoplastics (NPs) Increase the Toxicity of Metals in Fish Cell Lines

dc.contributor.author	González Fernández, Carmen
dc.contributor.author	Díaz Baños, F. Guillermo
dc.contributor.author	Esteban Abad, María Ángeles
dc.contributor.author	Cuesta, Alberto
dc.contributor.department	Química Física
dc.date.accessioned	2024-02-06T11:25:55Z
dc.date.available	2024-02-06T11:25:55Z
dc.date.created	2021
dc.date.issued	2021
dc.description	Copyright: © 2021 by the authors. Li‐ censee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and con‐ ditions of the Creative Commons At‐ tribution (CC BY) license (http://crea‐ tivecommons.org/licenses/by/4.0/).
dc.description.abstract	Nanoplastics (NPs) are one of the most abundant environment‐threatening nanomaterials on the market. The objective of this study was to determine in vitro if functionalized NPs are cytotoxic by themselves or increase the toxicity of metals. For that, we used 50 nm polystyrene nanoparticles with distinct surface functionalization (pristine, PS‐Plain; carboxylic, PS‐COOH; and amino PS‐NH2) alone or combined with the metals arsenic (As) and methylmercury (MeHg), which possess an environmental risk to marine life. As test model, we chose a brain‐derived cell line (SaB‐1) from gilthead seabream (Sparus aurata), one of the most commercial fish species in the Mediterranean. First, only the PS‐NH2 NPs were toxic to SaB‐1 cells. NPs seem to be internalized into the cells but they showed little alteration in the transcription of genes related to oxidative stress (nrf2, cat, gr, gsta), cellular protection against metals (mta) or apoptosis (bcl2, bax). However, NPs, mainly PSCOOH and PS‐NH2, significantly increased the toxicity of both metals. Since the coexistence of NPs and other pollutants in the aquatic environment is inevitable, our results reveal that the combined effect of NPs with the rest of pollutants deserves more attention.	es
dc.format	application/pdf	es
dc.format.extent	16	es
dc.identifier.doi	https://doi.org/10.3390/ijms22137141
dc.identifier.eisbn	International Journal of Molecular Science 2021, 22, 7141.	es
dc.identifier.issn	Print: 1661-6596
dc.identifier.issn	Electronic: 1422-0067
dc.identifier.uri	http://hdl.handle.net/10201/138714
dc.language	eng	es
dc.publisher	MDPI (Basel, Switzerland)	es
dc.relation	Ministerio de Ciencia e Innovación‐Agencia Estatal de Investigación (grant PID2019‐105522GB‐I00), Ministerio de Economía y Competitividad (MINECO; grant CTQ2017‐87708‐R) y Fundación Séneca (grant 20977/PI/18 y Grupo de Excelencia de la Región de Murcia 19883/GERM/15). C. González‐Fernández está contratada con el programa espanol Juan de la Cierva‐Incorporación JC2019‐040510‐I.	es
dc.relation.publisherversion	https://www.mdpi.com/1422-0067/22/13/7141
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Gilthead seabream	es
dc.subject	Cell lines	es
dc.subject	Nanoplastics	es
dc.subject	Metals	es
dc.subject	Fish	es
dc.subject.other	CDU::5 - Ciencias puras y naturales	es
dc.title	Functionalized Nanoplastics (NPs) Increase the Toxicity of Metals in Fish Cell Lines	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es