Publication: Comparison of histopathological features of Vibrio cholerae O1 El Tor and O139 Bengal infections in rabbit intestinal mucosa
Loading...
Date
2009
Authors
Amin, Atif ; Ali, Atif ; Kurunathan, Sinnah ; Cheong, Tan Gim ; Al-Jashamy, Karim Alwan ; Jaafar, Hasnan ; Zainuddin, Zainul F. ; Ravichandran, Manickam ; Lalitha, Pattabhiraman
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Vibrio cholerae is the causative agent of the
infectious disease, cholera. The bacteria adhere to the
mucosal membrane and release cholera toxin, leading to
watery diarrhea. There are >100 serovars of V. cholerae,
but the O1 and O139 serovars are the main causative
agents of cholera. The present study aimed to compare
the severity of intestinal mucosal infection caused by O1
El Tor and O139 V. cholerae in a rabbit ileal loop model.
The results showed that although the fluid accumulation
was similar in the loops inoculated with O1 and O139 V.
cholerae, the presence of blood was detected only in the
loops inoculated with the O139 serovar. Serosal
hemorrhage was confirmed by histopathological
examination and the loops inoculated with O139 showed
massive destruction of villi and loss of intestinal glands.
The submucosa and muscularis mucosa of the ileum
showed the presence of edema with congested blood
vessels, while severe hemorrhage was seen in the
muscularis propria layer. The loops inoculated with O1
El Tor showed only minimal damage, with intact
intestinal villi and glands. Diffuse colonies of the O139
serovar were seen to have infiltrated deep into the
submucosal layer of the intestine. Although the infection
caused by the O1 serovar was focal and invasive, it was more superficial than that due to O139, and involved
only the villi. These observations were confirmed by
immunostaining with O1 and O139 V. cholerae-specific
monoclonal antibodies. The peroxidase reaction
demonstrated involvement of tissues down to the
submucosal layer in O139 V. cholerae infection, while in O1 El Tor infection, the reaction was confined mainly to
the villi, and was greatly reduced in the submucosal
region. This is the first reported study to clearly
demonstrate the histopathological differences between
infections caused by the O139 Bengal and O1 El Tor
pathogenic serovars of V. cholerae.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.