DigitalUM :: Browsing by Subject "Rabbit"

Browsing by Subject "Rabbit"

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Open Access
Comparison of histopathological features of Vibrio cholerae O1 El Tor and O139 Bengal infections in rabbit intestinal mucosa
(Murcia : F. Hernández, 2009) Amin, Atif; Ali, Atif; Kurunathan, Sinnah; Cheong, Tan Gim; Al-Jashamy, Karim Alwan; Jaafar, Hasnan; Zainuddin, Zainul F.; Ravichandran, Manickam; Lalitha, Pattabhiraman
Vibrio cholerae is the causative agent of the infectious disease, cholera. The bacteria adhere to the mucosal membrane and release cholera toxin, leading to watery diarrhea. There are >100 serovars of V. cholerae, but the O1 and O139 serovars are the main causative agents of cholera. The present study aimed to compare the severity of intestinal mucosal infection caused by O1 El Tor and O139 V. cholerae in a rabbit ileal loop model. The results showed that although the fluid accumulation was similar in the loops inoculated with O1 and O139 V. cholerae, the presence of blood was detected only in the loops inoculated with the O139 serovar. Serosal hemorrhage was confirmed by histopathological examination and the loops inoculated with O139 showed massive destruction of villi and loss of intestinal glands. The submucosa and muscularis mucosa of the ileum showed the presence of edema with congested blood vessels, while severe hemorrhage was seen in the muscularis propria layer. The loops inoculated with O1 El Tor showed only minimal damage, with intact intestinal villi and glands. Diffuse colonies of the O139 serovar were seen to have infiltrated deep into the submucosal layer of the intestine. Although the infection caused by the O1 serovar was focal and invasive, it was more superficial than that due to O139, and involved only the villi. These observations were confirmed by immunostaining with O1 and O139 V. cholerae-specific monoclonal antibodies. The peroxidase reaction demonstrated involvement of tissues down to the submucosal layer in O139 V. cholerae infection, while in O1 El Tor infection, the reaction was confined mainly to the villi, and was greatly reduced in the submucosal region. This is the first reported study to clearly demonstrate the histopathological differences between infections caused by the O139 Bengal and O1 El Tor pathogenic serovars of V. cholerae.
Open Access
Cytomorphological changes in the rabbit oviductal epithelium after human chorionic gonadotropin treatment
(Murcia : F. Hernández, 1997) Bondi, A.M.; Gabrielli, M.G.; Marchetti, L.; Materazzi, G.; Menghi, Giovanna
An electron microscopic investigation was performed to examine the ultrastructural changes occurring in the rabbit oviductal epithelium after human chorionic gonadotropin (HCG) administration. Mainly, the non-ciliated secretory cells proved to be affected by the hormonal treatment which resulted in qualitative and quantitative modifications of the secretory patterns differently expressed in the ampulla and isthmus. Thus, morphological evidence of intense secretion was observed in both the oviduct regions at preovulatory stages. Following ovulation, timing of expression of active secretory patterns in the ampulla and isthmus correlated well with the rate of gamete transport and relative functional roles of the oviductal regions in the reproductive process. At present, HCG-induced changes concerning the ciliated cells seem to consist of the occurrence of secretory granules responsible for the appearance of "mixed cells".
Open Access
Development of germ cell neoplasia in situ in chinchilla rabbits
(Universidad de Murcia. Departamento de Biología Celular e Histología, 2016) Vigueras-Villaseñor, Rosa María; Montelongo Solís, Paola; Chávez-Saldaña, Margarita; Gutiérrez-Pérez, Oscar; Cortés Trujillo, Lucero; Rojas-Castañeda, Julio César
The present study was designed to describe the development of germ cell neoplasia in situ in Chinchilla rabbit by administration of estradiol. The study was performed in rabbits distributed into two groups: control and 17 β-estradiol. The determination of histological alterations and POU5F1 and c-kit proteins employed as biomarkers for the diagnosis of this neoplasia was carried out. Testicular descent and complete spermatogenesis were observed in the control group. The protein biomarkers were negative. However, in the rabbits treated with estradiol, the testes remained undescended with the gonocytes undifferentiated to spermatogonia. There were histological lesions owing to germ cell neoplasia in situ and positive to POU5F1 and c-kit proteins. These findings indicate that the chinchilla rabbit is an ideal model to study this neoplasia in which the histological characteristics and biomarkers of the disease could be clearly observed. Using this model we suggested that the persisting gonocytes could be responsible for the development of germ cell neoplasia in situ.
Open Access
Differential location and structural specificities of sialic acid-ß-D-Gal sequences belonging to sialoderivatives of rabbit oviduct under hormonal treatment
(Murcia : F. Hernández, 2004) Gabrielli, M.G.; Bondi, A.M.; Materazzi, G.; Menghi, Giovanna
Sialoderivatives expressed in the rabbit oviduct under hormonal treatment have been investigated in situ by lectin histochemistry with a view to specifying further regional and temporal specializations which enable ampulla and isthmus to play distinct roles in the reproductive events. Application of MAL II and SNA lectins allowed sialoglycoconjugates containing Sia(a2,3)Gal and Sia(a2,6)Gal groups to be discriminated. Sialic acid residues linked to Gal(ß1,3)-D-GalNAc sequences were identified using PNA combined with sialidase digestion. Information on structural features of sialic acids were acquired by deacetylation and differential oxidation pretreatments. In both oviductal portions, Sia(a2,6) groups were restricted to the luminal surface of the lining epithelium while Sia(a2,3) groups were specifically located in the secretory, non-ciliated cells. In the ampullary epithelium, non-acetylated sialic acids a2,3-linked to Gal(ß1,3)-D-GalNAc sequences were largely present. Only at ovulation time were sialic acid residues containing acetyl substituents on C4 also found. A great variety of sialic acids were found in the isthmic epithelium which showed the highest expression of acetylated forms at the first hours after the hormonal treatment. The heterogeneity of sialoderivatives differently expressed in the ampulla and isthmus as well as their distinct cycle-dependent modulation suggest that sialylated components may contribute to the molecular and functional specificities within the oviductal epithelium.
Open Access
Postnatal maturation of the parenchymal cell types in the rabbit pineal gland
(Murcia : F. Hernández, 1992) García-Mauriño, J.E.; Boya, J.
An ultrastructural study on the maturation of the parenchymal rabbit pineal cell types from the first postnatal day up to 120 days is presented. Two main cell types are distinguished from the first 24h of postnatal life. Pinealocytes of the types 1 and 11 display different developmental degrees. Both immature cell types are arranged in groups. In addition, type 11 pinealocytes form rosette-like structures. Both cell types progressively become isolated and display cell processes. The nucleus and the cytoplasm of type 1 pinealocytes are barely electrondense. During the postnatal period, the number of cytoplasmic organelles, cell processes and terminal clubs increase progressively. Terminal clubs are frequently seen near blood vessels. After 30 days, type 1 pinealocytes show characteristics of adult pinealocytes. However, the maturation of rnost type 1 pinealocytes does not complete until the 90th postnatal day. Type 11 pinealocytes present a fairly electrondense nucleus and cytoplasm. Mature forms can be seen after the 5th postnatal day. During the postnatal period, a close relationship is determined arnong type 11 pinealocytes and cell processes and terminal clubs of type 1 pinealocytes.
Open Access
Rabbit zona pellucida composition: A molecular, proteomic and phylogenetic approach
(2012) Stetson, I.; Chevret, P.; Lorenzo, P.L.; Ballesta, J.; Rebollar, P.G.; Gutiérrez-Gallego, R.; Izquierdo Rico, María José; Avilés Sánchez, Manuel; Moros Nicolás, Carla; Biología Celular e Histología
The zona pellucida (ZP) participates in sperm–egg interactions during the first steps of 22 fertilization. Recent studies have shown that the ZP matrix of oocytes in several species is 23 composed of four glycoproteins, designated as ZP1, ZP2, ZP3 and ZP4, rather than the three 24 described in mouse, pig and cow. In this study, investigations were carried out to unveil a Q425 fourth glycoprotein in the rabbit (Oryctolagus cuniculus) ZP. Using total RNA isolated from 26 rabbit ovaries, the complementary deoxyribonucleic acid (cDNA) encoding rabbit ZP1 was 27 amplified by reverse transcribed polymerase chain reaction (RT-PCR). The ZP1 cDNA 28 contains an open reading frame of 1825 nucleotides encoding a polypeptide of 608 amino 29 acid residues. The deduced amino acid sequence of rabbit ZP1 showed high identity with 30 other species: 70% identity with human and horse ZP1, and 67% identity with mouse and rat 31 ZP1. At the proteomic level, peptides corresponding to the four proteins were detected by 32 mass spectrometry. In addition, a molecular phylogenetic analysis of ZP1 showed that 33 pseudogenization of this gene has occurred at least four times during the evolution of 34 mammals. The data presented in this manuscript provide evidence, for the first time, that 35 the rabbit ZP is composed of four glycoproteins.
Open Access
Short-Term Effects of Deliberate Subparaneural or Subepineural Injections With Saline Solution or Bupivacaine 0.75% in the Sciatic Nerve of Rabbits
(Frontiers Media, 2020-05-12) Laredo Álvarez, Francisco Ginés; Belda Mellado, Eliseo; Soler Laguía, Marta; Gil Cano, Francisco; Murciano Pérez, José; Sánchez Campillo, Joaquín; Agut Giménez, Amalia; Anatomía y Anatomía Patológica Comparada
Background: Ultrasound (US)-guided techniques for peripheral nerve blockade have revealed that intraneural injections are relatively frequent and not necessarily associated with neurological deficits. Objectives: To evaluate the short-term effects of deliberate injections performed under direct vision in two different sites of the sciatic nerve (ScN). Material and Methods: Seventy-two New Zealand white rabbits randomly assigned to one of four experimental groups (n = 18) were employed. All procedures were conducted at a proximal femoral level where the ScN incorporates the common peroneal nerve and the tibial nerve (TN). Fixed volumes of 0.5ml of saline solution (ES group) or bupivacaine 0.75% (EB group) were administered extrafascicularly inside the paraneurium of the ScN or intrafascicularly (IS and IB groups) under the epineurium of the TN. Cross-sectional area (CSA) and relative echogenicity (RE) of the entire ScN were determined by US before injections, after injections, and at 3 and 7 days. ScN samples were obtained for structural and ultrastructural histopathological studies. Proprioceptive, sensorial, and motor function were clinically evaluated on a daily basis. Results: The CSA of the ScN increased significantly immediately after injections when compared with pre-injection values in all groups (p < 0.05). The RE of the ScN decreased in relation to pre-injection values in all groups (p < 0.05). The CSA and RE of the ScN returned to normal values 7 days after injections in almost all groups. Injected nerves showed histological signs of mild perineural inflammation. Histopathological scores were not significantly different between groups (p > 0.05). The architecture of the ScN was preserved in all rabbits at 3 days and in 31/32 rabbits at 7 days. A focal area of damaged nerve fibers with degeneration of the axons and myelin sheath affecting the TN was observed in one rabbit of the IB group. Nerve function was not clinically impaired in any case. Conclusion: Despite the lack of severe nerve disruption observed in most rabbits, the evidence of a focal area of damaged nerve fibers in one rabbit injected intrafascicularly with bupivacaine confirms that intrafascicular injections should be avoided as they may increase the risk of nerve damage.
Open Access
The effect of thymic inoculation to induce tolerance of allogeneic thyroid grafts in the outbred rabbit
(Murcia : F. Hernández, 1998) Torchia, M.G.; Aitken, R.M.; Thliveris, A.
Many studies have demonstrated that allograft tolerance can be achieved in inbred rats and mice following intrathymic injection of donor cells or antigen and treatment with antilymphocyte serum (ALS). In outbred dogs, xenografts, and inbred rat strains with major MHC antigen difference, tolerance has not similarly been induced. The focus of this study was to determine whether allogeneic thyroid graft tolerance could be achieved in outbred rabbits. In the experimental group (n=5), recipients received an intrathymic injection of donor lymphocytes and a single treatment of ALS. Controls (n=5) received intrathymic cell culture medium and ALS treatment. Donor-recipient allogenicity was monitored with mixed lymphocyte culture (MLC) over 18 weeks. Donor thyroid tissue was placed into recipient gluteal muscle fibres one week following the last MLC measurement. A third group of rabbits (n=4) received thyroid autografts without any other treatment. There were no differences in MLC stimulation indices (SI) between the control and experimental group nor did MLC (SI) change within groups. All thyroid autografts survived the two week monitoring period and demonstrated normal appearing thyroid follicles on histologic examination. All thyroid allografts showed severe acute rejection reactions on biopsy within one week. Further studies using outbred animals to examine the role of thymic inoculation are required to determine whether similar techniques might be successful in the human.
Open Access
The effects of Eicosapentaenoic acid on the endothelium of the carotid artery of rabbits on a high-cholesterol diet
(Murcia : F. Hernández, 2010) Cayli, Sevil; Sati, Leyla; Seval-Celik, Yasemin; Altug Tuncer, M.; Yaymaci, Bengi; Berkman, Zafer; Altug, Tuncay; Demir, Ramazan
The preventive and therapeutic effects ofEicosapentaenoic acid (EPA) on diet-inducedhyperlipidemia in rabbits have been investigated.Eighteen New Zealand rabbits were randomly dividedinto three groups of 6 subjects each; experimental group-I (EG-I) was administered a cholesterol rich diet,experimental group-II (EG-II) was treated with EPA(300 mg/kg/d) following a cholesterol-rich diet and thecontrol group (CG) had a standard diet. Blood sampleswere collected at day 0 and at the 4th and 12th weeks ofEG-II to obtain serum levels of total cholesterol (TC),high density lipid-cholesterol (HDL-C), low densitylipid-cholesterol (LDL-C) and triglyceride (TG). Fromeach group tissue samples were collected from thecarotid artery for immunohistochemistry and electronmicroscopy. Our results showed that EPA couldsignificantly lower (p<0.001) serum TC, LDL-C, HDL-C and TG levels with a reduction of 35%; 55%; 44% and51%, respectively. Scanning and transmission electronmicroscopy results revealed that endothelial damage wasmore prominent in EG-I when compared to EG-II. Theruptured endothelial lining and damaged cellular surfacewas increased in EG-I when compared to EG-II.Ultrastructural observations showed that after EPAtreatment, the degeneration and cellular surface damageon the endothelium were also decreased. These biochemical and ultrastructural results suggestthat EPA is a potential drug which significantly lowersthe serum lipid profile and partially repairs endothelialdysfunction due to hyperlipidemia
Open Access
Three-dimensional architecture of the connective tissue core and surface structures of the lingual papillae in the rabbit
(Murcia : F. Hernández, 2002) Silva, M.C.P.; Watanabe, I.; Kronka, M.C.
Three-dimensional characteristics of the epithelial cell layer and connective tissue interface of the tongue were studied using scanning electron microscopy. In this study, the fragments of tongue were fi xed in m o d i fied Karnov s ky ’s fi x a t ive solution. Subsequently, the specimens were treated with 10% NaOH solution for 4-7 days at room temperature and postfixed in 1% OsO4 in 0.1M phosphate buffer (pH 7.4) for 2 hours at 4 °C. T h ey were dehydrated through a graded ethanol series, and critical-point dried with CO2. The specimens were coated with gold and observed in a scanning electron microscope, JEOL JSM-6100. The results show e d numerous papillae on the dorsal surface of the tongue divided into four groups (filiform, fungiform, foliate and vallate papillae). Filiform papillae are conically shaped; fungiform papillae have an irregular round surfa c e ; foliate papillae are oval in shape and have some parallel projections; and vallate papillae are located in the posterior part of the tongue and have a depression around the center. After the treatment with 10% NaOH solution, the original arrangements of connective papillae could be seen. This characteristic threedimensional distribution of the collagen fiber bundles is typical for each superficial papillae depending on whether it is filiform, fungiform, foliate or vallate.
Open Access
Ultrastructural changes in the synovial membrane in experimentally-induced osteoarthritis of rabbit knee joint
(Murcia : F. Hernández, 1992) Vasilev, V.; Merker, H. J.; Vidinov, N.
Rabbit knee joint osteoarthritis was induced by intraarticular injections of a 10% sterile NaCl solution. Within 30 days the synovial membrane had undergone hyperplasia resulting in activated synovial fibroblasts. Transitional forms of synoviocytes as well as activated synovial macrophages were a very common finding. At 60 days a thickening of the synovial intima was perceptible. Most of the synoviocytes were of the fibroblast type. Transitional cell forms abounded. An increase in collagen fibres and capillaries of the fenestrated type occurred in the intercellular spaces. In the deep layer collagen bundles had formed between which activated fibroblasts and macrophages were noticed. The described changes point to an active participation of the synovial membrane in the destruction of articular cartilage in osteoarthritis.

Browsing by Subject "Rabbit"

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