Acidophilic granulocytes of the marine fish gilthead seabream (Sparus aurata L.) produce interleukin-1b following infection with Vibrio anguillarum

Chaves-Pozo, Elena; Pelegrin, Pablo; García-Castillo, Jesús; García-Ayala, Alfonsa; Mulero, Victoriano; Meseguer, José

Publication:
Acidophilic granulocytes of the marine fish gilthead seabream (Sparus aurata L.) produce interleukin-1b following infection with Vibrio anguillarum

dc.contributor.author	Chaves-Pozo, Elena
dc.contributor.author	Pelegrin, Pablo
dc.contributor.author	García-Castillo, Jesús
dc.contributor.author	García-Ayala, Alfonsa
dc.contributor.author	Mulero, Victoriano
dc.contributor.author	Meseguer, José
dc.contributor.department	Bioquímica y Biología Molecular B e Inmunología
dc.date.accessioned	2024-01-30T11:05:30Z
dc.date.available	2024-01-30T11:05:30Z
dc.date.issued	2004
dc.description	©2004. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published, version of an Accepted Work that appeared in final form in Cell and Tissue Research. To access the final edited and published work see https://doi.org/10.1007/s00441-004-0875-9
dc.description.abstract	The fish immune response to Gram-negative bacteria is poorly understood. In this study, we use a monoclonal antibody (mAb) specific to acidophilic granulocytes from the marine fish gilthead seabream (Sparus aurata L.), together with an antiserum specific to interleukin-1b (IL-1b) from this species, in order to investigate whether these cells are involved in the immune response against the pathogenic bacterium Vibrio anguillarum and, in particular, in the production of the pro-inflammatory cytokine IL-1b. We found that gilthead seabream head- kidney, peritoneal exudate and peripheral blood leukocytes accumulated proIL-1b intracellularly when challenged in vitro with V. anguillarum, whereas only peritoneal exudate and blood leukocytes were able to accumulate proIL-1b following infection. Importantly, the blood leukocytes from infected animals that accumulated proIL-1b were shown to be the acidophilic granulocytes. A rapid mobilization of such cells from the head-kidney to the site of inflammation following infection with V. anguillarum was also observed.	es
dc.format	application/pdf	es
dc.format.extent	26	es
dc.identifier.doi	https://doi.org/10.1007/s00441-004-0875-9
dc.identifier.eisbn	Cell and Tissue Research, volumen 316, nº 2, año 2004, páginas 189-195.	es
dc.identifier.uri	http://hdl.handle.net/10201/138143
dc.language	eng	es
dc.publisher	Springer	es
dc.relation	Spanish Ministry of Science and Technology (BIO2001-2324-C02-02, AGL2002-03529), Spanish Ministry of Education, Culture and Sport, Fundación Seneca, Coordination Centre for Research (PI-51/ 00782/FS/01)	es
dc.relation.publisherversion	https://link.springer.com/article/10.1007/s00441-004-0875-9	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Cytokine	es
dc.subject	Interleukin-1β	es
dc.subject	Sparus aurata	es
dc.subject	Fish	es
dc.subject.other	CDU::6 - Ciencias aplicadas	es
dc.title	Acidophilic granulocytes of the marine fish gilthead seabream (Sparus aurata L.) produce interleukin-1b following infection with Vibrio anguillarum	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es