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A rational approach to improving the biotechnological production of taxanes in plant cell cultures of Taxus spp.

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dc.contributor.authorCusido, Rosa M.
dc.contributor.authorOnrubia, Miriam
dc.contributor.authorSabater Jara, Ana Belén
dc.contributor.authorMoyano, Elisabeth
dc.contributor.authorBonfill, Mercedes
dc.contributor.authorGoossens, Alain
dc.contributor.authorPedreño, María Angeles
dc.contributor.authorPalazón, Javier
dc.contributor.departmentBiología Vegetal
dc.date.accessioned2025-05-15T09:53:34Z
dc.date.available2025-05-15T09:53:34Z
dc.date.issued2014-03-27
dc.description© 2014 Elsevier Inc. This document is the Published Manuscript version of a Published Work that appeared in final form in Biotechnology Advances. To access the final edited and published work see https://doi.org/10.1016/j.biotechadv.2014.03.002
dc.description.abstractTaxol is a complex diterpene alkaloid scarcely produced in nature and with a high anticancer activity. Biotechnological systems for taxol production based on cell cultures of Taxus spp. have been developed, but the growing commercial demand for taxol and its precursors requires the optimization of these procedures. In order to increase the biotechnological production of taxol and related taxanes in Taxus spp. cell cultures, it is necessary not only to take an empirical approach that strives to optimize in-put factors (cell line selection, culture conditions, elicitation, up-scaling, etc.) and out-put factors (growth, production, yields, etc.), but also to carry out molecular biological studies. The latter can provide valuable insight into how the enhancement of taxane biosynthesis and accumulation affects metabolic profiles and gene expression in Taxus spp. cell cultures. Several rational approaches have focused on studying the transcriptomic profiles of key genes in the taxol biosynthetic pathway in Taxus spp. cell cultures treated with elicitors such as methyl jasmonate, coronatine and cyclodextrins in relation with the taxane pattern, production and excretion to the culture medium. These studies have provided new insights into the taxol biosynthetic pathway and its regulation. Additionally, identifying genes with low levels of expression even in the presence of elicitors, together with metabolomics studies, has shed light on the limiting steps in taxol biosynthesis and could help define suitable metabolic targets for engineering with the main aim of obtaining highly productive Taxus cultured cells. In this review, we have summarized the latest endeavors to enhance the molecular understanding of the action mechanism of elicitors in Taxus spp. cell cultures. Developments in the ongoing search for new and more effective elicitation treatments and the application of metabolic engineering to design new transgenic cell lines of Taxus with an improved capacity for taxane production are described.
dc.embargo.termsSi
dc.formatapplication/pdfes
dc.format.extent11
dc.identifier.citationBiotechnology Advances, 2014, Vol. 32, Issue 6, pp. 1157-1167
dc.identifier.doihttps://doi.org/10.1016/j.biotechadv.2014.03.002
dc.identifier.issnPrint: 0734-9750
dc.identifier.issnElectronic: 1873-1899
dc.identifier.urihttp://hdl.handle.net/10201/154671
dc.languageenges
dc.publisherElsevier
dc.relationSpanish MEC (BIO2011-29856-C01), Generalitat de Catalunya (2009SGR1217), Fundación Lavoisier (LAVOISIER-1/2013), Cost Action Plant Engine (FA1006).es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S073497501400038X
dc.rightsinfo:eu-repo/semantics/embargoedAccesses
dc.subjectTaxus spp
dc.subjectTaxol
dc.subjectTaxanes
dc.subjectPlant cell culture
dc.subjectTranscriptomics
dc.subjectMetabolic engineering
dc.subjectElicitation
dc.subjectMethyl jasmonate
dc.subjectCyclodextrins
dc.subjectCoronatine
dc.titleA rational approach to improving the biotechnological production of taxanes in plant cell cultures of Taxus spp.es
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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