Publication: Producción de metabolitos secundarios en cultivo de raíces in vitro y suspensiones celulares de Ipomoea carnea spp. carnea Jacq.
Authors
Rojas Idrogo, Consuelo ; Kato, Massuo J. ; Delgado-Paredes, Guillermo E. ; Segal Floh, Eny Iochevet ; Handro, Walter
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Publisher
Murcia: Servicio de Publicaciones de la Universidad de Murcia
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Description
Abstract
Ipomoea carnea spp. carnea Jacq. es un arbusto dominante en el
bosque estacionalmente seco del Perú y Ecuador. Los cultivos de
raíces fueron establecidos en medio MS con diferentes concentraciones
de AIB, sacarosa y combinaciones de AIB-sacarosa. Diversos
explantes obtenidos de plántulas in vitro y plantas silvestres se
cultivaron en diferentes combinaciones de 2,4-D, AIA, ANA y BAP,
para inducir callos friables, dependiendo del tipo de explante y los
reguladores de crecimiento. Suspensiones celulares se establecieron
a partir de callos y estudiaron en su crecimiento y acumulación
de metabolitos secundarios. Los metabolitos secundarios identificados
en cultivo de raíces y suspensiones celulares fueron fenoles,
diversos flavonoides y la cumarina escopoletina.
ABSTRACT: Ipomoea carnea spp. carnea Jacq. is a dominant shrub in the seasonally dry forest of Peru and Ecuador. The root cultures were established on MS medium with different concentrations of IBA, sucrose and combinations of IBA-sucrose. Several explants obtained from in vitro plantlets and wild plants were cultured in different combinations of 2,4-D, IAA, NAA and BAP in order to induce friable calluses, which was depended of explant type and plant growth regulators. From a callus culture, cell suspensions were established and their growth and metabolite accumulation studied. Secondary metabolites identified, in the root and cell suspension cultures, were phenols, flavonoids, and coumarin scopoletin
ABSTRACT: Ipomoea carnea spp. carnea Jacq. is a dominant shrub in the seasonally dry forest of Peru and Ecuador. The root cultures were established on MS medium with different concentrations of IBA, sucrose and combinations of IBA-sucrose. Several explants obtained from in vitro plantlets and wild plants were cultured in different combinations of 2,4-D, IAA, NAA and BAP in order to induce friable calluses, which was depended of explant type and plant growth regulators. From a callus culture, cell suspensions were established and their growth and metabolite accumulation studied. Secondary metabolites identified, in the root and cell suspension cultures, were phenols, flavonoids, and coumarin scopoletin
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