Publication:
Evaluation of distribution of emerging mycotoxins in human tissues: applications of dispersive liquid–liquid microextraction and liquid chromatography‑mass spectrometry

Simple item page
dc.contributor.authorCastell Martínez, Ana
dc.contributor.authorArroyo Manzanares, Natalia
dc.contributor.authorPalma Manrique, Rosa
dc.contributor.authorCampillo Seva, Natalia
dc.contributor.authorTorres, Carmen
dc.contributor.authorFenoll, José
dc.contributor.authorViñas López-Pelegrin, Pilar
dc.contributor.departmentQuímica Analítica
dc.date.accessioned2025-10-23T08:00:57Z
dc.date.available2025-10-23T08:00:57Z
dc.date.copyright© The Author(s) 2023
dc.date.issued2023-11-21
dc.description.abstractIn this work, a complete study of the distribution of emerging mycotoxins in the human body has been carried out. Specifically, the presence of enniatins (A, A1, B, B1) and beauvericin has been monitored in brain, lung, kidney, fat, liver, and heart samples. A unique methodology based on solid–liquid extraction (SLE) followed by dispersive liquid–liquid microextraction (DLLME) was proposed for the six different matrices. Mycotoxin isolation was performed by adding ultrapure water, acetonitrile, and sodium chloride to the tissue sample for SLE, while the DLLME step was performed using chloroform as extraction solvent. Subsequently, the analysis was carried out by high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC–MS/MS). The proposed method allowed limits of quantification (LOQs) to be obtained in a range of 0.001–0.150 ng/g, depending on the tissue and mycotoxin. The precision was investigated intraday and interday, not exceeding of 9.8% of relative standard deviation. In addition, trueness studies achieved 75 to 115% at a mycotoxin concentration of 25 ng/g and from 82 to 118% at 5 ng/g. The application of this methodology to 26 forensic autopsies demonstrated the bioaccumulation of emerging mycotoxins in the human body since all mycotoxins were detected in tissues. Enniatin B (ENNB) showed a high occurrence, being detected in 100% of liver (7 ± 13 ng/g) and fat samples (0.2 ± 0.8 ng/g). The lung had a high incidence of all emerging mycotoxins at low concentrations, while ENNB, ENNB1, and ENNA1 were not quantifiable in heart samples. Co-occurrence of mycotoxins was also investigated, and statistical tests were applied to evaluate the distribution of these mycotoxins in the human body.
dc.formatapplication/pdf
dc.identifier.citationAnalytical and Bioanalytical Chemistry, 2024, Vol. 416, pp. 449-459
dc.identifier.doihttps://doi.org/10.1007/s00216-023-05040-8
dc.identifier.eissn1618-2650
dc.identifier.issn1618-2642
dc.identifier.urihttp://hdl.handle.net/10201/168429
dc.languageeng
dc.publisherSpringer
dc.relationMinisterio de Ciencia e Innovación (MCIN) (Proyecto PID2021-123201NB-I00)
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s00216-023-05040-8
dc.rightsAttribution 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectEmerging mycotoxins
dc.subjectEnniatins
dc.subjectBeauvericin
dc.subjectBioaccumulation
dc.subjectHuman tissues
dc.subjectDLLME
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleEvaluation of distribution of emerging mycotoxins in human tissues: applications of dispersive liquid–liquid microextraction and liquid chromatography‑mass spectrometry
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
relation.isAuthorOfPublication1cbd3819-b58d-4f27-8714-d8941702c12e
relation.isAuthorOfPublicationb248fbac-12a2-425f-a31e-bf96a78661c6
relation.isAuthorOfPublication5265e3bc-1bd2-4594-bd49-7eec3161904d
relation.isAuthorOfPublication04c2972d-50f3-46da-bbde-2bd8a2f2f089
relation.isAuthorOfPublication.latestForDiscovery1cbd3819-b58d-4f27-8714-d8941702c12e
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Anal. Bioanal. Chem..pdf
Size:
1.33 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.37 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos