Publication:
Bottlenose dolphins (Tursiops truncatus) do also cast neutrophil extracellular traps against the apicomplexan parasite Neospora caninum

Simple item page
dc.contributor.authorVillagra-Blanco, R.
dc.contributor.authorSilva, L.M.R.
dc.contributor.authorAguilella-Segura, A.
dc.contributor.authorArcenillas Hernández, Irene
dc.contributor.authorMartínez Carrasco-Pleite, Carlos
dc.contributor.authorSeipp, A.
dc.contributor.authorGärtner, U.
dc.contributor.authorRuiz de Ybáñez Carnero, María del Rocío
dc.contributor.authorTaubert, A.
dc.contributor.authorHermosilla, C.
dc.contributor.departmentSanidad Animal
dc.date.accessioned2024-01-08T08:55:24Z
dc.date.available2024-01-08T08:55:24Z
dc.date.issued2017-12
dc.description© 2017 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology. This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/). This document is the published version of a published Work that appeared in final form in International Journal for Parasitology: Parasites and Wildlifees
dc.description.abstractNeutrophil extracellular traps (NETs) are web-like structures composed of nuclear DNA decorated with histones and cytoplasmic peptides which antiparasitic properties have not previously been investigated in cetaceans. Polymorphonuclear neutrophils (PMN) were isolated from healthy bottlenose dolphins (Tursiops truncatus), and stimulated with Neospora caninum tachyzoites and the NETs-agonist zymosan. In vitro interactions of PMN with the tachyzoites resulted in rapid extrusion of NETs. For the demonstration and quantification of cetacean NETs, extracellular DNA was stained by using either Sytox Orange® or Pico Green®. Scanning electron microscopy (SEM) and fluorescence analyses demonstrated PMN-derived release of NETs upon exposure to tachyzoites of N. caninum. Co-localization studies of N. caninum induced cetacean NETs proved the presence of DNA adorned with histones (H1, H2A/H2B, H3, H4), neutrophil elastase (NE), myeloperoxidase (MPO) and pentraxin (PTX) confirming the molecular properties of mammalian NETosis. Dolphin-derived N. caninum-NETosis were efficiently suppressed by DNase I and diphenyleneiodonium (DPI) treatments. Our results indicate that cetacean-derived NETs represent an ancient, conserved and relevant defense effector mechanism of the host innate immune system against N. caninum and probably other related neozoan parasites circulating in the marine environment.es
dc.formatapplication/pdfes
dc.format.extent8es
dc.identifier.doihttps://doi.org/10.1016/j.ijppaw.2017.09.002
dc.identifier.eisbnInternational Journal for Parasitology: Parasites and Wildlife Volume 6, Issue 3, December 2017, Pages 287-294es
dc.identifier.issn2213-2244
dc.identifier.urihttp://hdl.handle.net/10201/137040
dc.languageenges
dc.publisherElsevieres
dc.relationSin financiación externa a la Universidades
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S2213224417300639?via%3Dihubes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectTursiops truncatuses
dc.subjectCetaceanses
dc.subjectNeutrophil extracellular trapses
dc.subjectInnate immunityes
dc.subjectNeospora caninumes
dc.titleBottlenose dolphins (Tursiops truncatus) do also cast neutrophil extracellular traps against the apicomplexan parasite Neospora caninumes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Bottlenose dolphins neutrophil extracellular traps against Neospora caninum.pdf
Size:
1.2 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.26 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Informes o documentos de trabajo