Publication: The Influence of a membrane environment on the structure and stability of a prokaryotic potassium channel, KcsA
Authors
Encinar Hidalgo, José Antonio ; Molina Gallego, María Luisa ; Poveda Larrosa, José Antonio ; Barrera Olivares, Francisco Nicolás ; Renart Pérez, María Lourdes ; Fernández Carvajal, Asia María ; González Ros, José Manuel
item.page.secondaryauthor
item.page.director
Publisher
Elsevier
publication.page.editor
publication.page.department
DOI
https://doi.org/10.1016/j.febslet.2005.08.038
item.page.type
info:eu-repo/semantics/article
Description
© 2005 Federation of European Biochemical Societies. This document is the Published version of a Published Work that appeared in final form in FEBS Letters. To access the final edited and published work see https://doi.org/10.1016/j.febslet.2005.08.038
Abstract
The lack of a membrane environment in membrane protein crystals is considered one of the major limiting factors to fully imply X-ray structural data to explain functional properties of ion channels [Gulbis, J.M. and Doyle, D. (2004) Curr. Opin. Struct. Biol. 14, 440–446]. Here, we provide infrared spectroscopic evidence that the structure and stability of the potassium channel KcsA and its chymotryptic derivative 1–125 KcsA reconstituted into native-like membranes differ from those exhibited by these proteins in detergent solution, the latter taken as an approximation of the mixed detergent-protein crystal conditions.
publication.page.subject
Citation
FEBS Letters, 2005, Vol. 579, Issue 23,pp. 5199-5204
item.page.embargo
Collections
Ir a Estadísticas
Sin licencia Creative Commons.