Publication: lmmunohistochemical analysis of adrenal proliferation and corticosterone expression in experimental adrenal regeneration
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Date
1995
Authors
Sarría, R. ; Losada, J. ; Bueno-López, J.L.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The proliferative activity, the organization
and the corticosterone expression of adrenocortical cells
in an experimental adrenal regeneration process after the
transplantation of neonatal adrenal glands to adult hosts
was investigated.
Three days after transplantation, the medullar and
the innermost adrenocortical cells of the neonatal
adrenal glands showed degenerative and necrotic
changes due to the lack of vascular supply. The
remaining outermost adrenocortical cells did not display
any PCNA immunoreaction. The first PCNA expression,
pointing out the beginning of the proliferative cycle, was
observed in a 45.4% of the adrenocortical cells, one
week after transplantation. After three weeks, severa1
regenerated adrenocortical nodules with a bigger size
than the one observed in the previous periods were seen.
In these nodules, while the outermost adrenocortical
cells were disposed in parallel to the capsule or in
rounded groups, the bulk of the regenerated mass width
was composed of cells forming longitudinal cords.
PCNA immunoreaction was almost exclusively
restricted to subcapsular cells (62.5%) and to cells of the
outermost portion of the cords (32.5%), the global
percentage of PCNA immunopositive cells being 18.4%.
Twelve weeks after transplantation, regenerated adrenocortical cells were arranged in three layers:
glomerulosa, fasciculata and reticularis. Only 1.85% of
the adrenocortical cells were PCNA immunopositive.
Although in the early stages of the regeneration
process, al1 the adrenocortical cells, both proliferating
and non proliferating cells expressed corticosterone, a
restriction of this immunoreactivity to the zonae
fasciculata and reticularis was observed when cell
zonation was apparent.
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