Publication:
In vitro effectiveness of recombinant human lactoferrin and its hydrolysate in alleviating LPS-induced inflammatory response

Simple item page
dc.contributor.authorEsmat, Aly
dc.contributor.authorDarwish, Aliaa Ali
dc.contributor.authorLópez Nicolás, Rubén
dc.contributor.authorRos Berruezo, Gaspar
dc.contributor.authorFrontela Saseta, María del Carmen
dc.contributor.departmentTecnología de Alimentos, Nutrición y Bromatología
dc.date.accessioned2026-07-01T10:35:58Z
dc.date.available2026-07-01T10:35:58Z
dc.date.copyright© 2017 Elsevier Ltd
dc.date.issued2017-12-14
dc.description.abstractThis study aimed to evaluate the potential anti-inflammatory role of the most produced form of lactoferrin expressed in various expression systems (Fe-saturated recombinant human Lf, rhLf) and its hydrolysate in concentrations resembles that found in mature human milk. Co-culture model consisted of CaCo-2 and RAW 246.7 cell lines was used to evaluate the potential anti-inflammatory activity of rhLf and its hydrolysate. During this experiment, CaCo-2 monolayer permeability and integrity was assayed through the measurement of transepithelial electrical resistance (TEER values). Also, the production of reactive oxygen species (ROS), nitric oxide (NO) and different cytokines (IL-8, IL-1β, IL-6, IL-10, IL-12p70, and TNF-α) were measured. The treatment with rhLf and its hydrolysate protected the monolayer integrity against LPS effect and reduced IL-8 and ROS production. This effect was dependent on the dose and 2 mg mL−1 of rhLf hydrolysate was more effective. The addition of rhLf and its hydrolysate to infant formula is a prominent step towards improving both infant formula functionality and newborn health. Thus, these functional ingredients could be incorporated in infant foods. In this context, ongoing researches are conducted to clarify this effect whether by using synthetic peptides or by using LPS-sepsis animal.
dc.formatapplication/pdf
dc.format.extent7
dc.identifier.citationFood Research International
dc.identifier.doihttps://doi.org/10.1016/j.foodres.2017.12.029
dc.identifier.eissn1873-7145
dc.identifier.issn0963-9969
dc.identifier.urihttp://hdl.handle.net/10201/240801
dc.languageeng
dc.publisherElsevier
dc.relationAgencia Española de Cooperación Internacional para el Desarrollo (no. 0000595994)
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0963996917308839?via%3Dihub
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectLactoferrin
dc.subjectLactoferrin hydrolysate
dc.subjectAnti-inflammatory effect
dc.subjectCaCo-2
dc.subjectRAW 246.7 macrophage
dc.subjectLipopolysaccharide, IL-8
dc.subjectROS production
dc.subjectPeptide fractions
dc.subject.odsObjetivo 3: Salud
dc.titleIn vitro effectiveness of recombinant human lactoferrin and its hydrolysate in alleviating LPS-induced inflammatory response
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublicationes
relation.isAuthorOfPublication6d5e0bf5-ef34-4006-afba-2462c3cb77f4
relation.isAuthorOfPublication25d4c680-1c6b-45f9-864d-bd7cfef1b64a
relation.isAuthorOfPublication12c1d05c-bf0e-4c4c-bd5a-16814b8541e9
relation.isAuthorOfPublication.latestForDiscovery6d5e0bf5-ef34-4006-afba-2462c3cb77f4
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Aly et al., Food Res Int., 2019.pdf
Size:
487.69 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.37 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos