Publication: Scanning electron microscopic study of natural killer cell-mediated cytotoxicity
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Date
1990
Authors
Fernández-Segura, E. ; García, J.M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The interaction between human natural
killer (NK) celis and NK-susceptible target cells, as well
as the mechanism involved in target cell lysis, were
studied with scanning electron microscopy (SEM). Low
density human peripheral blood lymphocytes, highly
enriched with large granular lymphocytes (LGL), were
used as effector cells, and K562-cells were used as
NK-susceptible target ceiis. The surface features of
LGLINK cells were examined under SEM. In the area of
interaction, NWtarget-cell conjugates showed microvilli
andor filipodia, and extensive areas of intercellular
contact. In addition, the effector cells in some NWtargetcell
conjugates were polarized toward the target cell.
Changes in target cell surface features included loss of
microvilli, large surface blebs and the appearance of
small pore-like lesions on the cell membrane. Our
findings show that target cell lysis occurred by apoptosis
and plasma membrane lesions analogous to those seen
during complement-mediated cytotoxicity.
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