Publication: Novel features of neurodegeneration
in the inner retina of early diabetic rats
Authors
Énzsöly, Anna ; Szabó, Arnold ; Szabó, Klaudia ; Szél, Ágoston ; Németh, János ; Lukáts, Ákos
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
https://doi.org/10.14670/HH-11-602
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info:eu-repo/semantics/article
Description
Abstract
The literature indicates that in diabetes
retinal dysfunctions related to neural retinal alterations
exist prior to clinically detectable vasculopathy. In a
previous report, a detailed description about the
alteration of the outer retina was given, where diabetic
degeneration preceded apoptotic loss of cells (Enzsöly et
al., 2014). Here, we investigated the histopathology of
the inner retina in early diabetes using the same
specimens. We examined rat retinas with immunohistochemistry and Western blotting, 12 weeks after
streptozotocin induction of diabetes. Glial reactivity was
observed in all diabetic retinal specimens; however, it
was not detectable all over the retina, but appeared in
randomly arranged patches, with little or no glia
activation in between. Similarly, immunoreactivity of
parvalbumin (staining mostly AII amacrine cells) was
also decreased only in some regions. We propose that
these focal changes appear prior to affecting the whole
retina and overt loss of cells. In contrast to these, most
other markers used (calretinin, recoverin, tyrosin
hydroxylase anti-Brn-3a and also calbindin in the optic
part of the retina) did not show any major alterations in
the intensity of immunoreactivity or in the number of
stained elements. Interestingly, under diabetic
conditions, the labeling pattern of PKC-α and calbindin
in the ciliary retina showed a clear resemblance to the pattern described during development. This observation
is in line with our previous study, reporting an increase
in the number of dual cones, coexpressing two
photopigments, which is another common feature with
developing retinas. These data may indicate a previously
uninvestigated regenerative capacity in diabetic retina
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