Publication:
Lycopene overproduction and in situ extraction in organic-aqueous culture systems using a metabolically engineered Escherichia coli

Simple item page
dc.contributor.authorDe Diego Puente, María Teresa
dc.contributor.authorGallego Jara, Julia
dc.contributor.authorDel Real, Álvaro
dc.contributor.authorÉcija Conesa, Ana
dc.contributor.authorManjón Rubio, Arturo
dc.contributor.authorCánovas Diaz, Manuel
dc.contributor.departmentBioquímica y Biología Molecular B e Inmunología
dc.date.accessioned2023-12-12T23:11:57Z
dc.date.available2023-12-12T23:11:57Z
dc.date.issued2015
dc.description©2015. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by /4.0/ This document is the Published, version of a Published Work that appeared in final form in AMB Express. To access the final edited and published work see https://doi.org/10.1186/s13568-015-0150-3
dc.description.abstractLycopene is an important compound with an increasing industrial value. However, there is still no biotechnological process to obtain it. In this study, a semi-continuous system for lycopene extraction from recombinant Escherichia coli BL21 cells is proposed. A two-phase culture mode using organic solvents was found to maximize lycopene production through in situ extraction from cells. Within the reactor, three phases were formed during the process: an aqueous phase containing the recombinant E. coli, an interphase, and an organic phase. Lycopene was extracted from the cells to both the interphase and the organic phase and, consequently, thus enhancing its production. Maximum lycopene production (74.71 ± 3.74 mg L−1 ) was obtained for an octane-aqueous culture system using the E. coli BL21LF strain, a process that doubled the level obtained in the control aqueous culture. Study of the interphase by transmission electron microscopy (TEM) showed the proteo-lipidic nature and the high storage capacity of lyco‑ pene. Moreover, a cell viability test by flow cytometry (CF) after 24 h of culture indicated that 24 % of the population could be re-used. Therefore, a batch series reactor was designed for semi-continuous lycopene extraction. After five cycles of operation (120 h), lycopene production was similar to that obtained in the control aqueous medium. A final specific lycopene yield of up to 49.70 ± 2.48 mg g−1 was reached at 24 h, which represents to the highest titer to date. In conclusion, the aqueous-organic semi-continuous culture system proposed is the first designed for lycopene extraction, representing an important breakthrough in the development of a competitive biotechnological process for lycopene production and extraction.
dc.formatapplication/pdfes_ES
dc.format.extent11
dc.identifier.doihttps://doi.org/10.1186/s13568-015-0150-3
dc.identifier.eisbn65es_ES
dc.identifier.eisbn5es_ES
dc.identifier.eisbnAMB Expresses_ES
dc.identifier.issn2191-0855
dc.identifier.urihttp://hdl.handle.net/10201/136584
dc.languageenges_ES
dc.relation.isreferencedbyED_IDENTRADA=1275
dc.rightsinfo:eu-repo/semantics/openAccess*
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectLycopene
dc.subjectMetabolically engineered Escherichia coli
dc.subjectFed-batch culture
dc.subjectOrganic-aqueous culture system
dc.titleLycopene overproduction and in situ extraction in organic-aqueous culture systems using a metabolically engineered Escherichia colies_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
AMBE-S-15-00050.pdf
Size:
1.59 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.26 KB
Format:
Plain Text
Description:
Written by org.dspace.content.LicenseUtils
Download
Collections
Artículos