Publication: Human eosinophils in vitro. An ultrastructural morphology primer
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Date
1994
Authors
Dvorak, A. M. ; Ishizaka, T.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
An ultrastructural morphological primer of
human eosinophils is presented. Mature and immature
eosinophils. obtained from peripheral blood and bone
marrow, as well as activated tissue eosinophils are all
used to illustrate the various morphologies assumed by
eosinophils in vivo. The various ultrastructural changes
expressed by this cell lineage in vivo reflect the impact of
differentiation, maturation, activation, secretion, and cell
injury on morphology. Nearly all of the changes
described in vivo are also evident in eosinophils arising
in in vitro systems. We review published studies of these
culture systems, which have been supplemented with
various conditioned media containing naturally
occurring growth factor(s) that are permissive (or not
permissive) for eosinophils or with the recombinant
growth factors, IL-5 or IL-3. These sti~diesw ere helpful
in the recognition of eosinophil-promoting, -sustaining
and -activating properties of human IL-3 and IL-5.
Moreover, mature and immature eosinophils were shown
to release a granule matrix protein - eosinophil
peroxidase (EPO) - by its transport in small cytoplasmic
vesicles, a process termed piecemeal degranulation
(PMD). accounting for the gradual emptying of granule
contents in the absence of granule fusions to the plasma
membrane. Also presented are eosinophil morphologies
that occur in vitro in suspension cultures of human cord
blood supplemented with the c-kit ligand from various
sources. The wide variety of eosinophil subcellular
changes in the c-kit ligand-supplemented cultures, like
the changes of which eosinophils are capable in vivo,
reflects the processes of differentiation, maturation,
activation, secretion and cell injury. Presentation of this
ultrastructural morphological primer of human
eosinophils in vitro should enable investigators to
recognize eosinophils in all of their diverse morphologic
forms i n cultures that contain differentiating and
Offprint requests to: Ann M. Dvorak. M.D.. Department of Pathology.
Beth lsrael Hospital. 330 Brookline Avenue. Boston. MA 02215, USA
functioning rnenibers of other lineages, also present in ckit
ligand-supplemented cultures. These lineages include
mast cells, basophils, neutrophils. monocytes, macrophages.
megakaryocytes, and endothelial cells.
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