In vitro immune-depression and anti-inflammatory activities of cantharidin on gilthead seabream (Sparus aurata) leucocytes activated by λ-carrageenan

Campos-Sánchez, Jose Carlos; Guardiola, Francisco A.; Esteban Abad, María Ángeles

Publication:
In vitro immune-depression and anti-inflammatory activities of cantharidin on gilthead seabream (Sparus aurata) leucocytes activated by λ-carrageenan

dc.contributor.author	Campos-Sánchez, Jose Carlos
dc.contributor.author	Guardiola, Francisco A.
dc.contributor.author	Esteban Abad, María Ángeles
dc.contributor.department	Biología Celular e Histología
dc.date.accessioned	2023-11-22T07:34:02Z
dc.date.available	2023-11-22T07:34:02Z
dc.date.created	2023-11-21
dc.description.abstract	Cantharidin is a natural compound with known therapeutic applications in humans. However, its properties have hardly been studied in fish. The aim of this study was to investigate the in vitro effects of cantharidin on gilthead seabream (Sparus aurata) head kidney leucocytes (HKL) stimulated with λ-carrageenan. Thus, HKLs were incubated for 24 h with cantharidin (0, 2.5 and 5 μg mL-1) and λ-carrageenan (0 and 1,000 μg mL-1) and cell viability and morphology, immunological parameters and several selected genes related to inflammation and apoptosis were evaluated. The results showed that cantharidin only decreased HKL viability when incubated with the highest dose (5 μg mL-1) and in combination with λ-carrageenan. As for the immunological parameters studied, cantharidin increased the peroxidase activity of HKLs only when incubated in combination with λ-carrageenan, but inhibited the respiratory burst and phagocytic activities even after being previously increased by λ-carrageenan. Furthermore, cantharidin induced morphological changes in HKLs with apoptotic and vacuolization signs that were enhanced when incubated in combination with λ-carrageenan. Considering the analysis of gene expression in HKLs, although λ-carrageenan up-regulated the expression of the proinflammatory gene il1b, cantharidin down-regulated its expression in HKLs exposed to both λ-carrageenan and cantharidin. In addition, cantharidin up-regulated casp3 and casp9 expression, which was down-regulated in HKLs incubated with both molecules. This combination also up-regulated casp1 expression. The present results provide detailed insight into the immune-depressive and anti-inflammatory properties of cantharidin on immune cells, which could be of interest to the aquaculture sector.	es
dc.format	application/vnd.ms-excel	es
dc.format.extent	5	es
dc.identifier.uri	http://hdl.handle.net/10201/135930
dc.language	eng	es
dc.relation	This research forms part of the ThinkInAzul programme supported by MCIN with funding from European Union Next Generation EU (PRTR-C17. I1) and by the Comunidad Autónoma de la Región de Murcia-Fundación Séneca (Spain).	es
dc.relation.ispartof	ThinkInAzul programme
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Cantharidin	es
dc.subject	λ-Carrageenan	es
dc.subject	Gilthead seabream (Sparus aurata)	es
dc.subject	Inflammation; Aquaculture	es
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología	es
dc.title	In vitro immune-depression and anti-inflammatory activities of cantharidin on gilthead seabream (Sparus aurata) leucocytes activated by λ-carrageenan	es
dc.type	info:eu-repo/semantics/dataset	es
dspace.entity.type	Publication	es