Microglial reaction in focal cerebral ischaemia
induced by intra-carotid homologous clot injection

Ng, Y.-K.; Ling, E.-A.

Publication:
Microglial reaction in focal cerebral ischaemia induced by intra-carotid homologous clot injection

dc.contributor.author	Ng, Y.-K.
dc.contributor.author	Ling, E.-A.
dc.date.accessioned	2020-09-04T14:29:05Z
dc.date.available	2020-09-04T14:29:05Z
dc.date.issued	2001
dc.description.abstract	This study examined the microglial reaction in a simulated thrombo-embolus ischaemia in rats given an intracarotid injection of a suspension of homologous blood clot. All rats including the controls receiving vehicle injection were perfused at 5 hours, and 1, 3 and 7 days post-operation. The brains were removed and processed for immunohistochemistry using a panel of monoclonal antibodies: OX-42, OX-18 and OX-6 for labeling of microglia. In rats given saline injection OX42 immunoreactive microglial cells were observed to be distributed quite evenly throughout the whole brain. When injection of clot suspension was given, microglial cells responded vigorously, particularly in the ipsilateral hippocampus. Microglial reaction was also detected in the ipsilateral cerebral cortex, caudate as well as septal nuclei. The majority of the detected reactive microglial cells were hypertrophied showing thick or stout processes. Some rod-like and amoeboid microglia were also observed. Rarely did the reactive microglia express OX-6 immunoreactivity. All microglial cells were unreactive for OX-18. The actual mechanisms leading to the microglial activation as well as functions of reactive microglia in focal cerebral ischaemia remain speculative. In the absence of direct evidence, it could only be suggested that they may act as sensor cells for detection of subtle alterations in the microenvironment, probably in response to focal ischaemia and/or leakage of serumderived factors induced by thrombo-embolus stroke.	es
dc.format	application/pdf	es
dc.format.extent	8	es
dc.identifier.eisbn	Histology and Histopathology, Vol. 16, n.º 1 (2001)	es
dc.identifier.issn	1699-5848
dc.identifier.issn	0213-3911
dc.identifier.uri	http://hdl.handle.net/10201/95713
dc.language	eng	es
dc.publisher	F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología	es
dc.relation	Sin financiación externa a la Universidad	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 International	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Homologous clot	es
dc.subject	Focal ischaemia	es
dc.subject	Microglial reaction	es
dc.subject	Brain	es
dc.subject	Rats	es
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología	es
dc.title	Microglial reaction in focal cerebral ischaemia induced by intra-carotid homologous clot injection	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es

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