Publication: Microglial reaction in focal cerebral ischaemia
induced by intra-carotid homologous clot injection
Loading...
Date
2001
Authors
Ng, Y.-K. ; Ling, E.-A.
item.page.secondaryauthor
item.page.director
Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
This study examined the microglial reaction
in a simulated thrombo-embolus ischaemia in rats given
an intracarotid injection of a suspension of homologous
blood clot. All rats including the controls receiving
vehicle injection were perfused at 5 hours, and 1, 3 and 7
days post-operation. The brains were removed and
processed for immunohistochemistry using a panel of
monoclonal antibodies: OX-42, OX-18 and OX-6 for
labeling of microglia. In rats given saline injection OX42 immunoreactive microglial cells were observed to be
distributed quite evenly throughout the whole brain.
When injection of clot suspension was given, microglial
cells responded vigorously, particularly in the ipsilateral
hippocampus. Microglial reaction was also detected in
the ipsilateral cerebral cortex, caudate as well as septal
nuclei. The majority of the detected reactive microglial
cells were hypertrophied showing thick or stout
processes. Some rod-like and amoeboid microglia were
also observed. Rarely did the reactive microglia express
OX-6 immunoreactivity. All microglial cells were
unreactive for OX-18. The actual mechanisms leading to
the microglial activation as well as functions of reactive
microglia in focal cerebral ischaemia remain speculative.
In the absence of direct evidence, it could only be
suggested that they may act as sensor cells for detection
of subtle alterations in the microenvironment, probably
in response to focal ischaemia and/or leakage of serumderived factors induced by thrombo-embolus stroke.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. CC BY 4.0