Publication: Characterization of the early pathology of cochlear stereocilia in four inbred mouse strains with progressive hearing loss
Authors
Liu, Xiang ; Xie, Yi ; Huang, Shanshan ; Xu, Ang ; Zhao, Mengmeng ; Kang, Xiaoxia ; Yan, Aiwei ; Li, Ping ; Jin, Changzhu ; Han, Fengchan
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Publisher
Universidad de Murcia. Departamento de BiologĂa Celular e HistologĂa
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DOI
DOI: 10.14670/HH-18-086
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info:eu-repo/semantics/article
Description
Abstract
Objective. Inbred strains of mice offer
promising models for understanding the genetic basis of
age-related hearing loss (AHL). NOD/LtJ, A/J, DBA/2J
and C57BL/6J mice are classical models of age-related
hearing loss and exhibit early onset of pathology of
AHL. This study was carried out to characterize the
early pathology of cochlear stereocilia in the four mouse
strains with age-related hearing loss.
Methods. The structural features of stereocilia in
NOD/LtJ, A/J, DBA/2J and C57BL/6J mice were
observed by scanning electron microscopy (SEM) at age
of 2, 4, 6 or 8, and 10 or 12 weeks. Meanwhile, auditoryevoked brainstem response (ABR) and distortion product
otoacoustic emission (DPOAE) amplitudes of the mice
were measured at various intervals (3, 4, 6, 8, 10 and 12
weeks of age).
Results. The ABR thresholds in NOD/LtJ, A/J and
DBA/2J mice increased with age from 3 to 12 weeks.
DPOAE amplitudes in NOD/LtJ, A/J, DBA/2J mice
were very low at 4 weeks and became negative at 8
weeks at f2 frequency of 17 672 Hz. In addition to the
progressive hearing loss, the four mouse strains
displayed early onset (at 2 weeks of age) and progressive
degeneration of stereocilia in hair cells.
Conclusion. Early degeneration of stereocilia
contributes to the functional impairment of hair cells and
hearing loss in NOD/LtJ, A/J, DBA/2J and C57BL/6J mice.
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Citation
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