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An ideal spacing is required for the control of Class II CRP-dependent promoters by the status of CRP K100

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dc.contributor.authorEcija Conesa, Ana
dc.contributor.authorGallego Jara, Julia
dc.contributor.authorLozano-Terol, Gema
dc.contributor.authorBrowning, Douglas F.
dc.contributor.authorBusby, Steve J.W.
dc.contributor.authorWolfe, Alan J.
dc.contributor.authorCánovas Díaz, Manuel
dc.contributor.authorDe Diego Puente, María Teresa
dc.contributor.departmentBioquímica y Biología Molecular B e Inmunología
dc.date.accessioned2021-11-03T17:19:34Z
dc.date.available2021-11-03T17:19:34Z
dc.date.issued2020-11-11
dc.description.abstractTranscription activation by the Escherichia coli CRP at Class II promoters is dependent on direct interactions between RNA polymerase and CRP, therefore the spatial proximity between both proteins plays a significant role in the ability of CRP to activate transcription. Using both in vivo and in vitro techniques, here we demonstrate that the CRP K100 positive charge, adjacent to AR2, is required for full promoter activity when CRP is optimally positioned. Accordingly, K100 mediated activation is very position-dependent and our data confirm that the largest impact of the K100 status on transcription activation occurs when the spacing between the CRP binding site and the A2 of the -10 element is 22 bp. From the results of this study and the progress in the understanding about open complex DNA scrunching, we propose that CRP-dependent promoters should now be numbered by the distance from the centre of the DNA site for CRP and the most highly conserved base at position 2 of the -10 hexamer in bacterial promoters.es
dc.formatapplication/pdfes
dc.format.extent26es
dc.identifier.doi10.1093/femsle/fnaa164.
dc.identifier.eisbnFEMS Microbiol Lett. (2020) 367(20):fnaa164es
dc.identifier.issn0378-1097
dc.identifier.urihttp://hdl.handle.net/10201/113632
dc.languageenges
dc.relationNombre del proyecto: Desvelando la dinámica del acetiloma para biología sintética en E. coli. Código: RTI2018-094393-B-C21. Agencia/entidad financiadora: Ministerio de Ciencia, Innovación y Universidades. Ámbito Nacional. Proyectos I+D+i - Modalidades «Retos Investigación» (con financiación FEDER). Convocatoria 2018. Nombre del proyecto: Biotecnología de sistemas para la mejora de bioprocesos de producción de proteínas recombinantes en E. coli: integración de la regulación transcripcional y post-traduccional del metabolismo de carbono y nitrógeno. Código: 19236/PI/14. Agencia/entidad financiadora: Fundación Séneca. Ámbito regional. Ayudas a la realización de proyectos para el desarrollo de investigación científica y técnica por grupos competitivos. Convocatoria 2018.es
dc.relation.publisherversionhttps://academic.oup.com/femsle/article-abstract/367/20/fnaa164/5936555?redirectedFrom=fulltextes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectEscherichia colies
dc.subjectcyclic AMP receptor protein (CRP)es
dc.subjecttranscription activationes
dc.subjectclass II CRP-dependent promoterses
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísicaes
dc.titleAn ideal spacing is required for the control of Class II CRP-dependent promoters by the status of CRP K100es
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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