Micafungin enhances the human macrophage response to Candida albicans through β-glucan exposure

Guirao-Abad, José Pedro; Sánchez-Fresneda, Ruth; Machado, Francisco; Argüelles, Juan Carlos; Martínez-Esparza Alvargonzález, María Concepción

Publication:
Micafungin enhances the human macrophage response to Candida albicans through β-glucan exposure

dc.contributor.author	Guirao-Abad, José Pedro
dc.contributor.author	Sánchez-Fresneda, Ruth
dc.contributor.author	Machado, Francisco
dc.contributor.author	Argüelles, Juan Carlos
dc.contributor.author	Martínez-Esparza Alvargonzález, María Concepción
dc.contributor.department	Bioquímica y Biología Molecular B e Inmunología
dc.date.accessioned	2024-06-26T11:56:33Z
dc.date.available	2024-06-26T11:56:33Z
dc.date.issued	2018-04-26
dc.description	© 2018 American Society for Microbiology. This document is the Published version of a Published Work that appeared in final form in Antimicrobial Agents and Chemotherapy. To access the final edited and published work see https://doi.org/10.1128/aac.02161-17
dc.description.abstract	Micafungin belongs to the antifungal family of echinocandins, which act as noncompetitive inhibitors of the fungal cell wall β-1,3-d-glucan synthase. Since Candida albicans is the most prevalent pathogenic fungus in humans, we study the involvement of micafungin in the modulation of the inflammatory response developed by human tissue macrophages against C. albicans The MIC for micafungin was 0.016 μg/ml on the C. albicans SC5314 standard strain. Micafungin induced a drastic reduction in the number of exponential SC5314 viable cells, with the fungicidal effect being dependent on the cellular metabolic activity. Notably, micafungin also caused a structural remodelling of the cell wall, leading to exposure of the β-glucan and chitin content on the external surface. At the higher doses used (0.05 μg/ml), the antifungal also induced the blowing up of budding yeasts. In addition, preincubation with micafungin before exposure to human tissue macrophages enhanced the secretion of tumor necrosis factor alpha (TNF-α), interleukin-17A (IL-17A), and IL-10 cytokines. Our results strongly suggest that in C. albicans treatment with micafungin, in addition to having the expected toxic antifungal effect, it potentiates the immune response, improving the interaction and activation of human macrophages, probably through the unmasking of β-glucans on the cell wall surface.	es
dc.format	application/pdf	es
dc.format.extent	9	es
dc.identifier.citation	Antimicrobial Agents and Chemotherapy 2018 May 62(5): e02161-17
dc.identifier.doi	https://doi.org/10.1128/AAC.02161-17
dc.identifier.issn	Print: 0066-4804
dc.identifier.issn	Electronic: 1098-6596
dc.identifier.uri	http://hdl.handle.net/10201/142714
dc.language	eng	es
dc.publisher	American Society for Microbiology	es
dc.relation	J.P.G.-A. and R.S.-F. received a partial fellowship from Cespa, Servicios Públicos de Murcia, S.A. (Murcia, Spain) and Vitalgaia España, S.L.	es
dc.relation.publisherversion	https://journals.asm.org/doi/10.1128/aac.02161-17
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Candida albicans	es
dc.subject	β-Glucan	es
dc.subject	Antifungal	es
dc.subject	Chitin	es
dc.subject	Cytokine	es
dc.subject	Macrophage	es
dc.subject	Micafungin	es
dc.title	Micafungin enhances the human macrophage response to Candida albicans through β-glucan exposure	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
relation.isAuthorOfPublication	79449fb4-f715-4255-a064-bfecc4b502de
relation.isAuthorOfPublication.latestForDiscovery	79449fb4-f715-4255-a064-bfecc4b502de