Publication: Immunoreactivity of Lewis blood group
and mucin peptide core antigens: Correlations
with grade of dysplasia and malignant transformation
in the colorectal adenoma-carcinoma sequence
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Date
2002
Authors
Baldus, S.E. ; Hanisch, F.G. ; Pütz, C. ; Flucke, U. ; Mönig, S.P. ; Schneider, P.M. ; Thiele, J. ; Hölscher, A.H. ; Dienes, H.P.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Previous studies on the immunoreactivity of
various mucin peptide and carbohydrate antigens in
neoplastic colorectal tissues led to at least in part
contradictory results. Therefore, we investigated a series
of 42 adenomas and 44 carcinomas applying monoclonal
antibodies (mabs) directed against Lewis blood group
antigens (sialyl-Lea, Lex, sialyl-Lex, Ley) as well as
mucin peptide cores (MUC1, MUC2 and MUC5AC) by
i m m u n o h i s t o c h e m i s t r y. A statistically significant
positive correlation between the development of highgrade
dysplasia in colorectal adenomas and the
immunoreactivity of Le y and MUC1 epitopes was
observed, whereas MUC2 exhibited a significant
negative correlation. The reactivity of the other epitopes
did not show an association with the progression of
malignant transformation. Colorectal carcinomas were
subdivided according to their histopathological subtype.
The immunohistochemical staining resulted in a
significantly stronger MUC2 reactivity of mucinous vs.
tubular adenocarcinomas. Immunoreactivity of the
MUC1-specific mab, which does not react with the fully
glycosylated peptide core, showed a statistically nonsignificant
inverse tendency, whereas all carbohydrate
antigens displayed a strong expression in both tumor
subtypes. Furthermore, correlations between mucin
peptide and carbohydrate epitope labelling were
evaluated. Progression of the adenoma-carcinoma
sequence was accompanied by an increase of Ley as well
as MUC1 antigen and an increase of all Lewis antigens
compared to MUC2 immunoreactivity. On the other
hand, mucinous carcinomas exhibited an inverse pattern.
In conclusion, these results demonstrate that Ley a n d
MUC1 immunoreactivity correlate with malignant transformation in the colorectum, whereas MUC2
represents a marker for low-grade dysplasia and the
subtype of mucinous carcinomas.
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