Publication: Nrf1 is time-dependently expressed
and distributed in the distinct cell types
after trauma to skeletal muscles in rats
Authors
Zhang, Shu-Tao ; Zhao, Rui ; Ma, Wen-Xiang ; Fan, Yan-Yan ; Guan, Wen-Zheng ; Wang, Jiao ; Ren, Peng ; Zhong, Kun ; Yu, Tian-Shui ; Pi, Jing-Bo ; Guan, Da-Wei
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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Description
Abstract
Our goal was to elucidate the dynamic
expression and distribution of the nuclear factor
erythroid-derived factor 2-related factor 1 (Nrf1) by
immunohistochemistry, Western blotting, and real-time
PCR during wound healing of contused skeletal muscle
in rats. An animal model of skeletal muscle contusion
was established in 40 Sprague-Dawley male healthy rats.
Samples were taken at 6 h, 12 h, 1 day, 3 days, 5 days, 7
days, 10 days, and 14 days post-injury, respectively (5
rats in each posttraumatic interval). 5 rats were
employed as control. A weak immunoreactivity of Nrf1
was observed in the sarcoplasm and nuclei of normal
myofibers in control rats. Prominent immunostaining for
Nrf1 was seen in a large number of polymorphonulcear
cells, round-shaped mononuclear cells and spindleshaped
fibroblastic cells, and regenerated multinucleated
myotubes in the injured tissue. Subsequently,
neutrophils, macrophages and myofibroblasts were
identified as expressing Nrf1 by double immunofluorescent
procedures. By real-time PCR analysis, Nrf1
expression was up-regulated and peaked at inflammatory
phase. The expression tendency was also confirmed by
Western blot. In conclusion, Nrf1 is time-dependently
expressed in certain cell types, such as neutrophils,
macrophages, myofibroblasts and regenerated
multinucleated myotubes, suggesting that Nrf1 may
modulate oxidative stress response and regeneration
after trauma to skeletal muscles.
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