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Pig in vitro fertilization: where are we and where do we go?

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dc.contributor.authorRomar, Raquel
dc.contributor.authorCánovas, Sebastián
dc.contributor.authorMatás, Carmen
dc.contributor.authorGadea, Joaquín
dc.contributor.authorCoy, Pilar
dc.contributor.departmentFisiología
dc.date.accessioned2024-02-12T12:55:20Z
dc.date.available2024-02-12T12:55:20Z
dc.date.created2019
dc.date.issued2019
dc.description©2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Theriogenology. To access the final edited and published work see https://doi.org/10.1016/j.theriogenology.2019.05.045es
dc.description.abstractThe pig is an important livestock animal. Biotechnological interest in this species has increased due to its use, among others, in the generation of transgenic animals for use in biomedicine based on its greater physiological proximity to the human species than other large domestic animals. This development has paralleled an improvement in Assisted Reproduction Techniques (ART) used for this species. However, the ability to generate animals from embryos produced entirely in vitro is still limited and a wide margin for improvement remains. Here we review the procedures, additives, and devices used during pig in vitro fertilization (IVF), focusing on the main points of each step that have offered the best results in terms of increased efficiency of the system. The lack of standardized protocols and consensus on the parameters to be assessed makes it difficult to compare results across different studies, but some conclusions are drawn from the literature. We anticipate that new physiological protocols will advance the field of swine IVF, including induction of prefertilization ZP hardening with oviductal fluid, sperm preparation by swim-up method, increased viscosity through the addition of inert molecules or reproductive biofluids, and the incorporation of 3D devices. Here we also reflect on the need to expand the variables on which the efficiency of pig IVF is based, providing new parameters that should be considered to supply more objective and quantitative assessment of IVF additives and protocols.es
dc.formatapplication/pdfes
dc.format.extent48es
dc.identifier.citationTheriogenology
dc.identifier.doihttps://doi.org/10.1016/j.theriogenology.2019.05.045
dc.identifier.issn0093-691X
dc.identifier.issn1879-3231
dc.identifier.urihttp://hdl.handle.net/10201/139287
dc.languageenges
dc.relationSin financiación externa a la Universidades
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0093691X19301840es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectPiges
dc.subjectIn vitro fertilizationes
dc.subjectEfficiencyes
dc.subjectAdditiveses
dc.subjectMonospermyes
dc.subjectIn vitro productiones
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::612 - Fisiologíaes
dc.titlePig in vitro fertilization: where are we and where do we go?es
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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