Publication: Enhanced accumulation of phytosterols and phenolic compounds incyclodextrin-elicited cell suspension culture of Daucus carota
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Date
2016-06-15
Authors
Miras Moreno, Begoña ; Almagro Romero, Lorena ; Pedreño, María Angeles ; Sabater Jara, Ana Belén
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Publisher
Elsevier
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Description
© 2016 Elsevier Ireland Ltd. This document is the Published Manuscript version of a Published Work that appeared in final form in Plant Science: An international journal of experimental plant biology. To access the final edited and published work see https://doi.org/10.1016/j.plantsci.2016.06.008
Abstract
Inthis work, suspension-culturedcells of Daucus carota wereusedto evaluate the effect of -cyclodextrins
on the production of isoprenoid and phenolic compounds. The results showed that the phytosterols and
phenolic compounds were accumulated in the extracellular medium (15100 g L−1 and 477.46 g L−1,
respectively) in the presence of cyclodextrins. Unlike the phytosterol and phenolic compound content,
-carotene (1138.03 g L−1), lutein (25949.54 g L−1) and -tocopherol (8063.82 g L−1) chlorophyll
a (1625.13 g L−1) and b (9.958 (9958.33 g L−1) were mainly accumulated inside the cells. Therefore, cyclodextrins were able to induce the cytosolic mevalonate pathway, increasing the biosynthesis
of phytosterols and phenolic compounds, and accumulate them outside the cells. However, in the
absence of these cyclic oligosaccharidic elicitors, carrot cells mainly accumulated carotenoids through
the methylerythritol 4-phosphate pathway.
Therefore, the use of cyclodextrins would allow the extracellular accumulation of both phytosterols
andphenolic compounds bydiverting the carbonflux towards the cytosolicmevalonate/phenylpropanoid
pathway.
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