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Usefulness of PCR for monitoring benznidazole response in patients with chronic Chagas’ disease: a prospective study in a non-disease-endemic country

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dc.contributor.authorMurcia, Laura
dc.contributor.authorCarrilero, Bartolome
dc.contributor.authorMuñoz, M. Jose
dc.contributor.authorIborra, M. Asunción
dc.contributor.authorSegovia, Manuel
dc.contributor.departmentGenética y Microbiología
dc.date.accessioned2025-09-18T08:54:10Z
dc.date.available2025-09-18T08:54:10Z
dc.date.issued2010-06-11
dc.description© The Author 2010.___ This document is the published version of a published work that appeared in final form in Journal of Antimicrobial Chemotherapy ___ To access the final edited and published work see: https://doi.org/10.1093/jac/dkq201
dc.description.abstractObjectives: This prospective study focused on the evaluation of antiparasitic therapy in chronic Chagas’ disease, monitored by PCR. Patients and methods: One hundred and eighty-one patients, all seropositive for Trypanosoma cruzi infection, underwent specific chemotherapy with benznidazole. Twelve patients were classified as young (2–19 years), 122 patients were classified as adults (20–39 years) and 47 patients were classified as seniors (≥40 years). In total, 116 (64%) were asymptomatic and 65 (36%) were symptomatic. Patients were monitored for 90, 150 and 420 days after treatment by PCR and serology. Results: Before treatment, 68% had a positive PCR result. PCR positivity was significantly more frequent in patients aged 2–19 years (P¼0.0007) and in short-term immigrants (P¼0.0076). No differences in PCR sensitivity were observed between samples of patients with chronic symptomatic or indeterminate Chagas’ disease. All patients presented an early conversion of PCR results 90 days post-treatment. However, at the end of the follow-up period PCR had become positive in four patients (4 out of 58 patients; 6.9%) who had strictly adhered to the treatment and in one who did not complete the treatment. Only one patient showed an alternating PCR during follow-up. Conclusions: We conclude that PCR is a useful tool for the early detection of parasite susceptibility to benznidazole and for the post-treatment parasitological follow-up of patients with chronic Chagas’ disease.
dc.embargo.terms01-ene-2999
dc.formatapplication/pdf
dc.format.extent6
dc.identifier.citationJournal of Antimicrobial Chemotherapy 2010; 65: 1759–1764
dc.identifier.doihttps://doi.org/10.1093/jac/dkq201
dc.identifier.eissnElectronic.: 1460-2091
dc.identifier.issnPrint.: 0305-7453
dc.identifier.urihttps://hdl.handle.net/10201/158629
dc.languageeng
dc.publisherOxford University Press
dc.relationThis study was supported by the Spanish Ministry of Science and Innovation and the Instituto de Salud Carlos III within the Network of Tropical Diseases Research (RICET RD06/0021/1007) and the Project of Research in Health (PS09/01956).
dc.relation.publisherversionhttps://academic.oup.com/jac/article-abstract/65/8/1759/742378?redirectedFrom=fulltext
dc.rights.accessRightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectKinetoplast DNA
dc.subjectParasitological follow-up
dc.subjectTrypanosoma cruzi
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleUsefulness of PCR for monitoring benznidazole response in patients with chronic Chagas’ disease: a prospective study in a non-disease-endemic country
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
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