Publication: Molecular genetic approaches to microtubule-associated protein function
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Date
2000
Authors
Gonzalez-Billault, C. ; Ávila, J.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Protein function in vivo can be studied by
deleting (knock-out) the gene that encodes it, and search
for the consequences. This procedure involves different
technologies, including recombinant DNA procedures,
cell biology methods and histological and immunocytochemical
analysis.
In this work we have reviewed these procedures
when they have been applied to ascertain the function of
several microtubule-associated proteins. These proteins
have been previously involved, through in vitro
experiments, in having a role in the microtubule
stabilization. Here, we will summarize the generation
and characterization of different microtubule-associated
protein knock-out mice. Special attention will be paid to
MAPlB knock-out mice. Amongst the different MAPs
knock-out mice these show the strongest phenotype, the
most likely for being MAPlB, the MAP that is
expressed earliest in neurogenesis.
Molecular genetics could be considered as a valid
and useful procedure to truly establish the in vivo
functions of a protein, although it is necessary to be
aware of possible artifacts such as the generation of
some kinds of RNA alternative splicing. To avoid this
the best strategy to be used must consider the deletion of
the exon that contains the functional domains of the
protein.
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