Browsing by Subject "Knockout"

Now showing 1 - 3 of 3
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    Caracterización del ‘Knock out’ en Boxeo
    (Murcia: Servicio de Publicaciones de la Universidad de Murcia, 2016) Pic Aguilar, M.; Sánchez-López, C.R.; Blanco-Villaseñor, Ángel
    El objetivo de este trabajo es identificar las cuatro últimas acciones motrices emitidas (golpes) por boxeadores campeones del mundo de los pesos pesados y así poder caracterizar el ‘Knock out’ en boxeo. Para ello, hemos desarrollado una herramienta de observación que consta de cuatro criterios y 35 categorías. Para la selección de la muestra se tuvo en cuenta dos requisitos: haberse proclamado campeón del mundo del peso pesado durante el período que comprende 1921-2007 (desde Jack Dempsey hasta Ruslan Chagaev) y la disponibilidad digital de las imágenes para su aná- lisis. Se obtuvieron datos relativos a la secuencia de acciones motrices que anteceden a la finalización de los combates en boxeo, medido a través de los últimos cuatro golpes lanzados por el ganador. Los resultados del estudio muestran que el ‘Knock out’ en boxeo suele darse haciendo un uso mayoritario de ciertos golpes entre los contendientes, presentando diferencias significativas.
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    Molecular genetic approaches to microtubule-associated protein function
    (Murcia : F. Hernández, 2000) Gonzalez-Billault, C.; Ávila, J.
    Protein function in vivo can be studied by deleting (knock-out) the gene that encodes it, and search for the consequences. This procedure involves different technologies, including recombinant DNA procedures, cell biology methods and histological and immunocytochemical analysis. In this work we have reviewed these procedures when they have been applied to ascertain the function of several microtubule-associated proteins. These proteins have been previously involved, through in vitro experiments, in having a role in the microtubule stabilization. Here, we will summarize the generation and characterization of different microtubule-associated protein knock-out mice. Special attention will be paid to MAPlB knock-out mice. Amongst the different MAPs knock-out mice these show the strongest phenotype, the most likely for being MAPlB, the MAP that is expressed earliest in neurogenesis. Molecular genetics could be considered as a valid and useful procedure to truly establish the in vivo functions of a protein, although it is necessary to be aware of possible artifacts such as the generation of some kinds of RNA alternative splicing. To avoid this the best strategy to be used must consider the deletion of the exon that contains the functional domains of the protein.
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    Platelet glycoprotein Ibα supports experimental lung metastasis
    (National Academy of Sciences, 2007-05-22) Guerrero López, José Antonio; Jain, Shashank; Zuka, Masahiko; Liu, Jungling; Russell, Susan; Dent, Judith; Forsyth, Jane; Maruszak, Brigid; Gartner, T. Kent; Felding-Habermann, Brunhilde; Ware, Jerry; Medicina Interna; Facultad de Medicina
    The platelet paradigm in hemostasis and thrombosis involves an initiation step that depends on platelet membrane receptors binding to ligands on a damaged or inflamed vascular surface. Once bound to the surface, platelets provide a unique microenvironment supporting the accumulation of more platelets and the elaboration of a fibrin-rich network produced by coagulation factors. The platelet-specific receptor glycoprotein (GP) Ib-IX, is critical in this process and initiates the formation of a platelet-rich thrombus by tethering the platelet to a thrombogenic surface. A role for platelets beyond the hemostasis/thrombosis paradigm is emerging with significant platelet contributions in both tumorigenesis and inflammation. We have established congenic (N10) mouse colonies (C57BL/6J) with dysfunctional GP Ib-IX receptors in our laboratory that allow us an opportunity to examine the relevance of platelet GP Ib-IX in syngeneic mouse models of experimental metastasis. Our results demonstrate platelet GP Ib-IX contributes to experimental metastasis because a functional absence of GP Ib-IX correlates with a 15-fold reduction in the number of lung metastatic foci using B16F10.1 melanoma cells. The results demonstrate that the extracellular domain of the α-subunit of GP Ib is the structurally relevant component of the GP Ib-IX complex contributing to metastasis. Our results support the hypothesis that platelet GP Ib-IX functions that support normal hemostasis or pathologic thrombosis also contribute to tumor malignancy.