Publication: Cellular compartmentation of lysozyme and a-amylase in the mouse salivary glands. lmmunogold approaches at light and electron microscopy level
Loading...
Date
2000
Authors
Marchetti, L. ; Gabrielli, M.G. ; Materazzi, G. ; Menghi, Giovanna
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
Description
Abstract
The research was planned to study the
subcellular distribution of enzymatic secretory products
within the secretory structures of the mouse major
salivary glands at light and electron microscopy level by
immunogold silver stain (IGSS) technique and doublesided
post-embedding immunogold binding and silver
amplification in order to speculate about their
compartmentation. In particular, we experimented the
above immunogold labeling approaches to localize the
lysozyme and to verify its distribution patterns in
relation to another secretion enzyme, a-amylase.
Co-presence of lysozyme and a-amylase was
observed in the convoluted granular tubule cells of the
submandibular gland and in the demilunar cells of the
sublingual gland as well as in the electron-dense regions
of the mottled secretory granules in the parotid gland.
Exclusive binding patterns of lysozyme were observed
in the acinar cells of the submandibular and sublingual
glands where a-amylase did not occur.
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.