Browsing by Subject "Lysozyme"

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    Cellular compartmentation of lysozyme and a-amylase in the mouse salivary glands. lmmunogold approaches at light and electron microscopy level
    (Murcia : F. Hernández, 2000) Marchetti, L.; Gabrielli, M.G.; Materazzi, G.; Menghi, Giovanna
    The research was planned to study the subcellular distribution of enzymatic secretory products within the secretory structures of the mouse major salivary glands at light and electron microscopy level by immunogold silver stain (IGSS) technique and doublesided post-embedding immunogold binding and silver amplification in order to speculate about their compartmentation. In particular, we experimented the above immunogold labeling approaches to localize the lysozyme and to verify its distribution patterns in relation to another secretion enzyme, a-amylase. Co-presence of lysozyme and a-amylase was observed in the convoluted granular tubule cells of the submandibular gland and in the demilunar cells of the sublingual gland as well as in the electron-dense regions of the mottled secretory granules in the parotid gland. Exclusive binding patterns of lysozyme were observed in the acinar cells of the submandibular and sublingual glands where a-amylase did not occur.
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    Morphological heterogeneity of myeloperoxidasepositive granules in normal circulating neutrophils, an ultrastructural study by cryosection
    (Murcia : F. Hernández, 1998) Saito, N.; Sato, F.; Asaka, M.; Takemori, N.; Kohgo, Y.
    Ultrastructural localization of myeloperoxidase (MPO) in the granules of human circulating neutrophils was examined by cryosection. On careful comparison with the morphological characteristics of the granules by conventional transmission electron microscopic study, large MPO-positive granules were divided into five types by immunocryoultramicrotomy using monoclonal antibody. Double staining of MP0 and lactoferrin (or lysozyme) was also performed. Lactoferrin was generally detected in MPO-negative granules. Lysozyme immunostaining was present in MPO-positive and -negative granules. These data may suggest different functions among large MPO-positive granules of human circulating neutrophils.